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Simple and Fail-safe Method to Transform Miniprep Strain K12 Plasmid DNA Into Viable EHA105 Cells for Plant Genetic Transformation.
Bio Protoc
January 2025
Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya.
Agrobacterium-mediated gene transformation method is a vital molecular biology technique employed to develop transgenic plants. Plants are genetically engineered to develop disease-free varieties, knock out unsettling traits for crop improvement, or incorporate an antigenic protein to make the plant a green factory for edible vaccines. The method's robustness was validated through successful transformations, demonstrating its effectiveness as a standard approach for researchers working in plant biotechnology.
View Article and Find Full Text PDFGenome-Wide Analysis of the Hsf Gene Family in and Function in Thermotolerance.
Int J Mol Sci
December 2024
Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China.
Heat shock transcription factors (Hsfs) play an important role in response to high temperatures by binding to the promoter of the heat shock protein gene to promote its expression. As an important ornamental plant, the rose often encounters heat stress during the flowering process. However, there are few studies on the family in roses ().
View Article and Find Full Text PDFEngineered Phage Enables Efficient Control of Gene Expression upon Infection of the Host Cell.
Int J Mol Sci
December 2024
CAS Key Laboratory for Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Recently, we developed a spatial phage-assisted continuous evolution (SPACE) system. This system utilizes chemotaxis coupled with the growth of motile bacteria during their spatial range expansion in soft agar to provide fresh host cells for iterative phage infection and selection pressure for preserving evolved genes of interest carried by phage mutants. Controllable mutagenesis activated only in a subpopulation of the migrating cells is essential in this system to efficiently generate mutated progeny phages from which desired individuals are selected during the directed evolution process.
View Article and Find Full Text PDFEnhanced Mechanical and Acoustic Properties of Basalt Fiber/Polyurethane Composites by Silane Coupling Agents.
Polymers (Basel)
December 2024
School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China.
Modified basalt microfiber-reinforced polyurethane elastomer composites were prepared by a semi-prepolymer method with two different silane coupling agents (KH550 and KH560) in this study. Infrared spectroscopy was used to quantify the degree of microphase separation and analyze the formation of hydrogen bonding in polyurethane. The interfacial surface and the morphology of fibers and composites from tensile fracture were examined by a scanning electron microscope.
View Article and Find Full Text PDFRisk Factors for Mortality Among Older Adults with Hospital-Acquired Bloodstream Infections in the Intensive Care Unit: A Multicenter Cohort Study.
Infect Dis Ther
January 2025
Infectious Diseases Unit, Sheba Medical Center, Ramat-Gan, Israel.
Introduction: We aimed to investigate risk factors for mortality among older adults (≥ 75 years) with hospital-acquired bloodstream infections (HA-BSI) in the intensive care unit (ICU).
Methods: We included patients aged ≥ 75 years with HA-BSI in ICU from the EUROBACT-2 cohort (2019-2021). Univariable and multivariable analyses were conducted to identify predictors of 28-day mortality.
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