AI Article Synopsis
- This study reveals the main pathway for inactivating the plant hormone auxin, specifically indole-3-acetic acid (IAA), through the GH3-ILR1-DAO pathway.
- The process begins with GH3 enzymes converting IAA into storage forms (IAA-aspartate and IAA-glutamate), which can be reverted back to active IAA by ILR1/ILL enzymes.
- Additionally, DAO1 irreversibly oxidizes these storage forms into inactive compounds, highlighting the significance of this pathway for maintaining auxin levels during plant development.
Article Abstract
Inactivation of the phytohormone auxin plays important roles in plant development, and several enzymes have been implicated in auxin inactivation. In this study, we show that the predominant natural auxin, indole-3-acetic acid (IAA), is mainly inactivated via the GH3-ILR1-DAO pathway. IAA is first converted to IAA-amino acid conjugates by GH3 IAA-amidosynthetases. The IAA-amino acid conjugates IAA-aspartate (IAA-Asp) and IAA-glutamate (IAA-Glu) are storage forms of IAA and can be converted back to IAA by ILR1/ILL amidohydrolases. We further show that DAO1 dioxygenase irreversibly oxidizes IAA-Asp and IAA-Glu into 2-oxindole-3-acetic acid-aspartate (oxIAA-Asp) and oxIAA-Glu, which are subsequently hydrolyzed by ILR1 to release inactive oxIAA. This work established a complete pathway for the oxidative inactivation of auxin and defines the roles played by auxin homeostasis in plant development.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8608799 | PMC |
| http://dx.doi.org/10.1038/s41467-021-27020-1 | DOI Listing |
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