AI Article Synopsis
- Bone marrow niches, specifically endosteal and perivascular, are crucial for normal functions and are involved in conditions like cancer cell dormancy.
- Researchers developed a 3D in vitro model of human bone marrow using advanced microfluidic and stem cell technologies, effectively mimicking real-life bone marrow environments.
- This new model allows for better understanding of bone marrow functions and responses to drugs by capturing dynamic events at a high resolution.
Article Abstract
Bone marrow niches (endosteal and perivascular) play important roles in both normal bone marrow function and pathological processes such as cancer cell dormancy. Unraveling the mechanisms underlying these events in humans has been severely limited by models that cannot dissect dynamic events at the niche level. Utilizing microfluidic and stem cell technologies, we present a 3D in vitro model of human bone marrow that contains both the perivascular and endosteal niches, complete with dynamic, perfusable vascular networks. We demonstrate that our model can replicate in vivo bone marrow function, including maintenance and differentiation of CD34 hematopoietic stem/progenitor cells, egress of neutrophils (CD66b), and niche-specific responses to doxorubicin and granulocyte-colony stimulating factor. Our platform provides opportunities to accelerate current understanding of human bone marrow function and drug response with high spatial and temporal resolution.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10658812 | PMC |
| http://dx.doi.org/10.1016/j.biomaterials.2021.121245 | DOI Listing |
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