Starch-rich by-products could be efficiently exploited for polyhydroxyalkanoates (PHAs) production. Unfortunately, Cupriavidus necator DSM 545, one of the most efficient PHAs producers, is not able to grow on starch. In this study, a recombinant amylolytic strain of C. necator DSM 545 was developed for the one-step PHAs production from starchy residues, such as broken rice and purple sweet potato waste. The glucodextranase G1d from Arthrobacter globiformis I42 and the α-amylase amyZ from Zunongwangia profunda SM-A87 were co-expressed into C. necator DSM 545. The recombinant C. necator DSM 545 #11, selected for its promising hydrolytic activity, produced high biomass levels with noteworthy PHAs titers: 5.78 and 3.65 g/L from broken rice and purple sweet potato waste, respectively. This is the first report on the engineering of C. necator DSM 545 for efficient amylase production and paves the way to the one-step conversion of starchy waste into PHAs.
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http://dx.doi.org/10.1016/j.biortech.2021.126383 | DOI Listing |
Bioresour Technol
January 2025
Department of Biology, University of Padova, 35131 Padova, Italy. Electronic address:
The fermentation process in alcoholic beverage production converts sugars into ethanol and CO, releasing significant amounts of greenhouse gases. Here, Cupriavidus necator DSM 545 was grown autotrophically using gas derived from alcoholic fermentation, using a fed-batch bottle system. Nutrient starvation was applied to induce intracellular accumulation of poly(3-hydroxybutyrate) (PHB), a bioplastic polymer, for bioconversion of CO-rich waste gas into PHB.
View Article and Find Full Text PDFEnzyme Microb Technol
December 2024
CIRIAF, Interuniversity Research Centre on Pollution and Environment "M.Felli", University of Perugia, Via G. Duranti 67, Perugia 06125, Italy.
The extensive utilization of conventional plastics has resulted in a concerning surge in waste. A potential solution lies in biodegradable polymers mostly derived from renewable sources. Cupriavidus necator DSM 545 is a microorganism capable, under stress conditions, of intracellularly accumulating Poly(3-hydroxybutyrate) (PHB), a bio-polyester.
View Article and Find Full Text PDFACS Sustain Chem Eng
September 2024
BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottingham, Nottingham NG7 2RD, U.K.
Water Res
December 2023
Centro de Biocombustibles y Bioproductos, Instituto Tecnológico Agrario de Castilla y León (ITACyL), Polígono Agroindustrial del Órbigo p. 2-6, Villarejo de Órbigo 24358, León, Spain.
Biodegradable biopolymers, such as polyhydroxyalkanoates (PHAs), have emerged as an alternative to petrochemical-based plastics. The present work explores the production of PHAs based on the biotransformation of potato processing wastewater and addresses two different strategies for PHA recovery. To this end, culture conditions for PHA synthesis by Cupriavidus necator DSM 545 were optimized on a laboratory scale using a response surface methodology-based experimental design.
View Article and Find Full Text PDFBioresour Technol
December 2023
Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), University of A Coruña, Rúa da Fraga 10, Coruña 15008 A, Spain.
The environmental concerns regarding fossil plastics call for alternative biopolymers such as polyhydroxyalkanoates (PHAs) whose manufacturing costs are however still too elevated. Autotrophic microbes like Cupriavidus necator, able to convert CO and H into PHAs, offer an additional strategy. Typically, the preferred source for CO and H are expensive pure gases or syngas, which has toxic compounds for most PHAs-accumulating strains.
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