Engineering Cupriavidus necator DSM 545 for the one-step conversion of starchy waste into polyhydroxyalkanoates.

Bioresour Technol

Department of Agronomy Food Natural resources Animals and Environment (DAFNAE), Università degli Studi di Padova, Agripolis, Viale dell'Università 16, 35020 Legnaro, (PD), Italy.

Published: March 2022

Starch-rich by-products could be efficiently exploited for polyhydroxyalkanoates (PHAs) production. Unfortunately, Cupriavidus necator DSM 545, one of the most efficient PHAs producers, is not able to grow on starch. In this study, a recombinant amylolytic strain of C. necator DSM 545 was developed for the one-step PHAs production from starchy residues, such as broken rice and purple sweet potato waste. The glucodextranase G1d from Arthrobacter globiformis I42 and the α-amylase amyZ from Zunongwangia profunda SM-A87 were co-expressed into C. necator DSM 545. The recombinant C. necator DSM 545 #11, selected for its promising hydrolytic activity, produced high biomass levels with noteworthy PHAs titers: 5.78 and 3.65 g/L from broken rice and purple sweet potato waste, respectively. This is the first report on the engineering of C. necator DSM 545 for efficient amylase production and paves the way to the one-step conversion of starchy waste into PHAs.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2021.126383DOI Listing

Publication Analysis

Top Keywords

necator dsm
24
dsm 545
24
cupriavidus necator
8
one-step conversion
8
conversion starchy
8
starchy waste
8
phas production
8
545 efficient
8
broken rice
8
rice purple
8

Similar Publications

The fermentation process in alcoholic beverage production converts sugars into ethanol and CO, releasing significant amounts of greenhouse gases. Here, Cupriavidus necator DSM 545 was grown autotrophically using gas derived from alcoholic fermentation, using a fed-batch bottle system. Nutrient starvation was applied to induce intracellular accumulation of poly(3-hydroxybutyrate) (PHB), a bioplastic polymer, for bioconversion of CO-rich waste gas into PHB.

View Article and Find Full Text PDF

Poly(3-hydroxybutyrate) production for food packaging from biomass derived carbohydrates by cupriavidus necator DSM 545.

Enzyme Microb Technol

December 2024

CIRIAF, Interuniversity Research Centre on Pollution and Environment "M.Felli", University of Perugia, Via G. Duranti 67, Perugia 06125, Italy.

The extensive utilization of conventional plastics has resulted in a concerning surge in waste. A potential solution lies in biodegradable polymers mostly derived from renewable sources. Cupriavidus necator DSM 545 is a microorganism capable, under stress conditions, of intracellularly accumulating Poly(3-hydroxybutyrate) (PHB), a bio-polyester.

View Article and Find Full Text PDF

Stable Platform for Mevalonate Bioproduction from CO.

ACS Sustain Chem Eng

September 2024

BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), Biodiscovery Institute, School of Life Sciences, The University of Nottingham, Nottingham NG7 2RD, U.K.

Article Synopsis
  • The stability of plasmids in microbial cells is crucial for efficient industrial biocatalysis, as these multicopy systems offer better product outcomes compared to genomic integrations.
  • The study focuses on H16, a bacterium capable of converting inorganic carbon from CO fixation into valuable products, which has struggled with plasmid stability.
  • Researchers developed a plasmid addiction system that stabilized a multicopy plasmid, allowing H16 to successfully produce approximately 10 g/L of mevalonate with carbon yields around 25%, marking a record for C6 compounds from C1 feedstocks.
View Article and Find Full Text PDF

Biotransformation of starch-based wastewater into bioplastics: Optimization of poly(3-hydroxybutyrate) production by Cupriavidus necator DSM 545 using potato wastewater hydrolysate.

Water Res

December 2023

Centro de Biocombustibles y Bioproductos, Instituto Tecnológico Agrario de Castilla y León (ITACyL), Polígono Agroindustrial del Órbigo p. 2-6, Villarejo de Órbigo 24358, León, Spain.

Biodegradable biopolymers, such as polyhydroxyalkanoates (PHAs), have emerged as an alternative to petrochemical-based plastics. The present work explores the production of PHAs based on the biotransformation of potato processing wastewater and addresses two different strategies for PHA recovery. To this end, culture conditions for PHA synthesis by Cupriavidus necator DSM 545 were optimized on a laboratory scale using a response surface methodology-based experimental design.

View Article and Find Full Text PDF

Autotrophic production of polyhydroxyalkanoates using acidogenic-derived H and CO from fruit waste.

Bioresour Technol

December 2023

Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), University of A Coruña, Rúa da Fraga 10, Coruña 15008 A, Spain.

The environmental concerns regarding fossil plastics call for alternative biopolymers such as polyhydroxyalkanoates (PHAs) whose manufacturing costs are however still too elevated. Autotrophic microbes like Cupriavidus necator, able to convert CO and H into PHAs, offer an additional strategy. Typically, the preferred source for CO and H are expensive pure gases or syngas, which has toxic compounds for most PHAs-accumulating strains.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!