We have recently demonstrated that the activity of hexokinase 2 is dependent on the intracellular potassium ion (K) concentration ([K]). To analyze the K dependency of the cell metabolism in cell populations, we used an extracellular flux analyzer to assess oxygen consumption and acidification rates as well-established measures of oxidative- and glycolytic metabolic activities. This protocol describes in detail how a potential K sensitivity of the cell metabolism can be elucidated by extracellular flux analysis. For complete details on the use and execution of this protocol, please refer to Bischof et al. (2021).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585662 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.100876 | DOI Listing |
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