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Background: Colistin is classified as the highest priority and critically important antimicrobial for human medicine by WHO as it is the last resort agent for treatment of carbapenem-resistant Enterobacteriaceae in humans. Additional research is necessary to elucidate the genetic structure of resistance genes, commonly found on plasmids, using WGS.
Objectives: To map and compare the genetic characteristics of 35 -mediated colistin-resistant Enterobacteriaceae isolated from chicken meat to highlight the genetic variation of the containing plasmids.
Methods: Sequencing was performed using Illumina HiSeq2500, Novaseq6000 and ONT's GridION. GridION data was locally basecalled and demultiplexed using ONT's Albacore 2.3.4 followed by Porechop 2.3. Quality filtering was performed using Filtlong 2.0. Hybrid Assembly was performed using Unicycler 4.7. Plasmids were compared with reference sequences in plasmid-RefSeq and pATLAS.
Results: A total of 35 positive Enterobacteriaceae were investigated, which resulted in 34 qualitatively robust hybrid assemblies of 2 and 32 . was present in 33/34 isolates. One isolate contained an like resistance gene, due to a deletion of one codon. Two genes were located on the chromosome, while the majority of the genes were found on IncX4 type plasmids (=19). Almost all plasmids identified in this study were highly similar to plasmids found in human-derived strains.
Conclusions: The -containing plasmids from retail chicken show high sequence similarity to human plasmids, suggesting that this may be a contributor to the presence of colistin resistance in humans.
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http://dx.doi.org/10.1093/jacamr/dlab156 | DOI Listing |
Appl Microbiol Biotechnol
December 2024
State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Nonstructural protein 3C, a master protease of Picornaviridae, plays a critical role in viral replication by directly cleaving the viral precursor polyprotein to form the viral capsid protein and antagonizing the host antiviral response. Additionally, 3C protease, as a tool enzyme, is involved in regulating polyprotein expression. Here, the 3C mutant gene (3Cm), fused with a small ubiquitin-like modifier (SUMO) tag at the N-terminal and featuring a mutation at position 127, was inserted into the cold-shock plasmid pCold of Escherichia coli for expression.
View Article and Find Full Text PDFElife
December 2024
UPMC Hillman Cancer Center, Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, United States.
Immune checkpoint inhibitors (ICIs) and their combination with other therapies such as chemotherapy, fail in most cancer patients. We previously identified the PDZ-LIM domain-containing protein 2 (PDLIM2) as a bona fide tumor suppressor that is repressed in lung cancer to drive cancer and its chemo and immunotherapy resistance, suggesting a new target for lung cancer therapy improvement. In this study, human clinical samples and data were used to investigate genetic and epigenetic changes in lung cancer.
View Article and Find Full Text PDFJ Biochem Mol Toxicol
January 2025
Anqing Medical College Clinical Research Center, Anqing Municipal Hospital, Anqing, Anhui, P.R. China.
Our previous research identified that lncRNA PVT1 is upregulated in patients with IA. However, the precise functions of PVT1 in IA remain unclear. We compared the levels of PVT1, caspase-3, caspase-1, and NLRP3 in normal and IA patients.
View Article and Find Full Text PDFNat Sci Sleep
December 2024
Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People's Republic of China.
Background: OSA can cause cognitive impairment (CI). The aim of this study was to investigate whether miR-20a-5p in exosomes derived from bEnd3 cells with IH mediates intercellular crosstalk and induces CI through hippocampal neuronal cell pyroptosis.
Materials And Methods: BEnd3-derived exosomes were isolated from the normal oxygen control group (NC-EXOS) and IH group (IH-EXOS).
Heliyon
December 2024
Department of Environmental Engineering, Faculty of Engineering, Mersin University, 33343, Yenisehir, Mersin, Turkey.
Cerium oxide NPs (-CeO), with notable performance in various biological tests like redox activity, free radical scavenging, and biofilm inhibition, emerge as significant candidates to address issues in related areas. In this research, copper-decorated -CeO (Cu@-CeO) were first synthesized and then characterized using advanced techniques such as SEM-EDX, XRD, XPS, BET, and ICP-OES. The biochemical properties of the obtained Cu@-CeO nanostructure and its performance in polyethersulfone (PES) membranes were thoroughly investigated in this research study.
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