We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600229 | PMC |
| http://dx.doi.org/10.3389/fmicb.2021.749783 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Conductive Eutectogels Fabricated by Dialdehyde Xylan/Liquid Metal-Initiated Rapid Polymerization for Multi-Response Sensors and Self-Powered Applications.
ACS Nano
January 2025
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
Conductive eutectogels have emerged as candidates for constructing functional flexible electronics as they are free from the constraints posed by inherent defects associated with solvents and feeble network structures. Nevertheless, developing a facile, environmentally friendly, and rapid polymerization strategy for the construction of conductive eutectogels with integrated multifunctionality is still immensely challenging. Herein, a conductive eutectogel is fabricated through a one-step dialdehyde xylan (DAX)/liquid metal (LM)-initiated polymerization of a deep eutectic solvent.
View Article and Find Full Text PDFPrimary charge separation in Chloroflexus aurantiacus reaction centers at room temperature: ultrafast transient absorption measurements on Q-depleted preparations with native and chemically modified bacteriopheophytin composition.
Photosynth Res
February 2025
Institute of Basic Biological Problems of the Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Moscow Region, 142290, Russian Federation.
The initial electron transfer (ET) processes in reaction centers (RCs) of Chloroflexus (Cfl.) aurantiacus were studied at 295 K using femtosecond transient absorption (TA) difference spectroscopy. Particular attention was paid to the decay kinetics of the primary electron donor excited state (P) and the formation/decay of the absorption band of the monomeric bacteriochlorophyll a anion (B) at ~ 1035 nm, which reflects the dynamics of the charge-separated state PB.
View Article and Find Full Text PDFMetasurface with all-optical tunability for spatially-resolved and multilevel thermal radiation.
Nanophotonics
April 2024
Laser Micro/Nano-Fabrication Laboratory, School of Mechanical Engineering, Beijing Institute of Technology, Beijing 100081, China.
Manipulating the thermal emission in the infrared (IR) range significantly impacts both fundamental scientific research and various technological applications, including IR thermal camouflage, information encryption, and radiative cooling. While prior research has put forth numerous materials and structures for these objectives, the significant challenge lies in attaining spatially resolved and dynamically multilevel control over their thermal emissions. In this study, a one-step ultrafast laser writing technique is experimentally demonstrated to achieve position-selective control over thermal emission based on the phase-change material GeSbTe (GST).
View Article and Find Full Text PDFOxygen vacancy mediated Pd-SA/TiO single-atom catalyst created via ultra-fast one-step synthesis for enhanced CO photoreduction.
J Colloid Interface Sci
December 2024
Key Laboratory for Ultrafine Materials of Ministry of Education, Shanghai Environmental, Friendly Materials Technical Service Platform, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
Single-atom catalysts (SACs) have garnered considerable interest in the field of heterogeneous catalysis. This study detail the synthesis of single-atom Pd catalysts supported on metal oxides using the Flame Spray Pyrolysis (FSP) method. This technique allows for the preparation of TiO with abundant oxygen vacancies by incorporating a hydrogen-rich atmosphere at high temperature (>2000 K) utilizing the quenching ring, which enhances the catalyst synthesis process.
View Article and Find Full Text PDFLarge-scale synthesis and exciton dynamics of monolayer MoS on differently doped GaN substrates.
Nanophotonics
December 2023
Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China.
Mixed dimensional van der Waals heterostructure based on layered two-dimensional molybdenum disulfide (MoS) interfaced to gallium nitride (GaN) has attracted tremendous attention due to its unique properties and application in novel electronic, optoelectronic, and quantum devices. However, developing facile synthesis methods and insights into the exciton dynamics for this system still remains a major challenge. Here, a simple and cost-effective method is demonstrated for large-scale synthesis of monolayer MoS on differently doped GaN substrates.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!