AI Article Synopsis

  • Hematopoiesis is the production of blood cells from stem cells in the bone marrow, and treatments with Cyclophosphamide can suppress this process, but Korean Red Ginseng (KRG) and CCA are suggested to enhance the immune system.
  • Research methods included analyzing hematopoietic stem cells, immune cell types, and serum markers, and found that specific combinations of KRG and CCA improved HSCs and immune cell populations in mice.
  • The study concluded that a 3:2 ratio of KRG to CCA is the most effective in restoring HSC levels in bone marrow, highlighting its potential for immune recovery.

Article Abstract

Background: Hematopoiesis is the production of blood cells from hematopoietic stem cells (HSCs) that reside in the bone marrow. Cyclophosphamide (CTX) is a chemotherapy drug that suppresses the immune system. Korean Red Ginseng (KRG) and (CCA) have been traditionally used for boosting the immune system.

Methods: HSCs in the bone marrow, and immune cell subtype in splenocytes, PBMCs, and thymocytes were investigated. Serum levels of hematopoietic-related markers were analyzed using ELISA. Protein expression in spleen tissue was analyzed using western blot analysis. Hematoxylin & eosin staining in the femurs of mice were also conducted.

Results: The combination of KRG and CCA with a ratio of 3:2 increased HSCs, CD3 and CD8 T cells in the circulation, and CD3 T cells in the spleen. A ratio of 2:3 (KRG:CCA) increased the thymic regulatory T cells and recovered the CD3 T cells in the spleen and circulation while recovering proteins in the JAK-STAT pathway in the spleen. Overall, blood cell population and differentiating factors vital for cell differentiation were also significantly recovered by all combinations especially in ratios of 3:2 and 2:3.

Conclusion: A ratio of 3:2 (KRG:CCA) is the most ideal combination as it recovered the HSC population in the bone marrow of mice.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587481PMC
http://dx.doi.org/10.1016/j.jgr.2021.02.004DOI Listing

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