Chemical components of var. and their cytotoxic and antibacterial activities.

Nineteen compounds, including seventeen alkaloids O-methylarmepavine (), (S)-6-methoxy-1-(4-methoxybenzyl)-2-methyl -1,2,3,4-tetrahydroisoquinolin-7-ol (), (+)-(IR,laR)-lahydroxymagnocurarin (), (6R,6aS,P)-(+)-corydine (), (+)-N-methyllaurotetanine (), magnoflorine (), 3-hydroxy-1,2-dimethoxy-5-methyl-5H-dibenzoindol-4-one (), imperialine (), crispine B (), (S)-1-(3-methoxyphenyl)-N-methylpropan-2-amine (), methyl 2- (acetamino)benzoate (), 2-carboxyoxanilic acid methylester (), 4-[2-(methoxycarbonyl) anilino]-4-oxobutanoic acid methyl ester (), N-methylcorydaldine (), N-methyl-6,7- dimethoxyisoquinolone (), (5,6,7,8)-5-amino-(2Z,4Z)-1,2,3-trihybuta-2,4-dienyloxypentane- 6,7,8,9-tetraol (), nicotinic acid (), and two megastigmane type compounds, S(+)- dehydrovomifoliol () and megastigmane (), were isolated from the Aconitum barbatum var. puberulum Ledeb. Compounds and were isolated from this plant for the first time, of which compound was isolated from natural source for the first time. Cytotoxicity evaluation revealed that compound displayed mild cytotoxicity against the Hela cell lines (IC50 13.69 ± 0.036 μM). Antibacterial activity evaluation revealed that compounds and showed strong antibacterial activity against the Gram-positive bacterium, S. aureus.