Correlation of mutation with prognosis of docetaxel in non-small cell lung cancer.

Background: Clinical features of epidermal growth factor receptor () mutations have been commonly recognized in variant cancers. The role of mutations in non-small cell lung cancer (NSCLC) has spurred research and drug development efforts. However, there are still mutations that have not been widely reported, and their influences on NSCLC have not been fully elucidated; mutation is just one of them. The aim of this study was to investigate the correlation between mutation and the prognosis of chemotherapy in NSCLC.

Methods: A total of 54 patients with NSCLC were enrolled in this study. Immunohistochemical staining was used to detect the expression of . A DNA extraction kit (GeneRead DNA FFPE Kit) was used to extract total DNA from resected cancer tissues. Genomic DNA targets were amplified by polymerase chain reaction (PCR), and then the amplicons were purified and sequenced. Statistical methods were performed to detect the relationship between mutation and various clinicopathological features and the effect of mutation on the prognosis of chemotherapy.

Results: mutation did not show statistical significance, with high expression identified in 30 cases (P>0.05). Patients with mutation had a significantly favorable prognosis of docetaxel (P=0.032), and for patients treated with docetaxel, mutation was significantly correlated with better 5-year disease-free survival (DFS; P=0.026) and overall survival (OS; P=0.026). However, there was no statistical significance found between mutation and the prognosis of vinorelbine (P>0.05), and for patients treated with vinorelbine, mutation had no statistical significance with 5-year DFS (P>0.05) and OS (P>0.05).

Conclusions: mutation was remarkably correlated with the prognosis of docetaxel in NSCLC, which indicates that may be employed as a promising biomarker to identify individuals with better prognosis of docetaxel and as an antitumor target for NSCLC treatment.