AI Article Synopsis
Article Abstract
Infectious pancreatic necrosis (IPN) is a highly contagious disease of young salmonid fish and is one of the most severe economic diseases in aquaculture. In Turkey, an increase in infectious pancreatic necrosis virus (IPNV) outbreaks in freshwater rainbow trout have been reported in recent years. This study aimed to analyze the VP2 gene from recent IPNV isolates from Turkey to determine whether there are epidemiological links between IPNV isolates from rainbow trout (Oncorhynchus mykiss; 62) and sea bass (Dicentrarchus labrax; 1), wild turbot (Scophthalmus maximus; 1) and the environment in order to investigate potential wild and farmed fish interactions. In this study, 62 Turkish IPNV isolates collected over 10 years (2005-2014) from rainbow trout, sea bass and turbot were genotypically characterized. The phylogenetic analysis indicated that Turkish IPNV isolates are closely related to strains from Denmark, Iran and Spain and that all Turkish IPNV isolates belong to genogroup V, serotype A2 (Sp strain). Furthermore, low genetic diversity was found among the Turkish isolates (identity, 95.5%-100% nucleotides and 97.8%-100% amino acids). The result of the analysis of the amino acid residues found at positions 217, 221 and 247 (proline, threonine and alanine, respectively) could be associated with virulence.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/jfd.13558 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The genetics of resistance to infectious pancreatic necrosis virus in rainbow trout unveiled through survival and virus load data.
Front Genet
November 2024
Department of Breeding and Genetics, Nofima, Ås, Norway.
Infectious Pancreatic Necrosis virus (IPNV) is one of the major threats to the animal welfare and economy of the rainbow trout farming industry. Previous research has demonstrated significant genetic variation for resistance against IPNV. The main objective of the study was to investigate the genetic architecture of resistance against IPNV in rainbow trout fry.
View Article and Find Full Text PDFFirst Report of Bacterial Kidney Disease (BKD) Caused by in Chum Salmon () Farmed in South Korea.
Microorganisms
November 2024
Department of Aqualife Medicine, Chonnam National University, Yeosu 59626, Republic of Korea.
Article Synopsis
- * The infectious pancreatic necrosis virus (IPNV) was isolated from some fish, but also, bacteria causing typical bacterial kidney disease (BKD) were identified using DNA sequencing and specific culture methods.
- * Histopathological studies showed severe kidney damage, indicating that the mass mortality was linked to the bacterial infection rather than IPNV, marking the first BKD outbreak associated with this bacteria in farmed chum salmon in South Korea.
Effect of a loss of the mda5/ifih1 gene on the antiviral resistance in a Chinook salmon Oncorhynchus tshawytscha cell line.
PLoS One
October 2024
INRAE, UVSQ, VIM, Université Paris-Saclay, Jouy-en-Josas, France.
Cells are equipped with intracellular RIG-like Receptors (RLRs) detecting double stranded (ds)RNA, a molecule with Pathogen-Associated Molecular Pattern (PAMPs) generated during the life cycle of many viruses. Melanoma Differentiation-Associated protein 5 (MDA5), a helicase enzyme member of the RLRs encoded by the ifih1 gene, binds to long dsRNA molecules during a viral infection and initiates production of type I interferon (IFN1) which orchestrates the antiviral response. In order to understand the contribution of MDA5 to viral resistance in fish cells, we have isolated a clonal Chinook salmon Oncorhynchus tshawytscha epithelial-like cell line invalidated for the ifih1 gene by CRISPR/Cas9 genome editing.
View Article and Find Full Text PDFDevelopment of a loop-mediated isothermal amplification (LAMP) assay for rapid and visual detection of Anguillid herpesvirus 1.
J Virol Methods
December 2024
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China. Electronic address:
Article Synopsis
- * Anguillid herpesvirus 1 (AngHV) has been identified as the cause of these diseases, prompting the development of a new diagnostic tool.
- * A loop-mediated isothermal amplification (LAMP) assay was created to swiftly and accurately detect AngHV, proving to be over 100 times more sensitive than traditional PCR methods, making it a promising solution for early diagnosis in eel farms.
Evolution of an Extended Pathogenicity Motif in VP2 of Infectious Pancreatic Necrosis Virus Isolates from Farmed Rainbow Trout in Turkey.
Viruses
June 2024
Medizinische Hochschule Brandenburg Theodor Fontane, 01968 Senftenberg, Germany.
Infectious pancreatic necrosis virus (IPNV) causes economic losses with a highly variable mortality rate worldwide, especially in rainbow trout. The virus has a double-stranded bi-partite RNA genome designated segment A and B. New complete genome sequences of nine rainbow trout isolates from Turkey were determined and subjected to phylogenetic analysis, identifying all as genotype 5 (serotype Sp).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!