We used viral metagenomics and next-generation sequencing to identify a novel strain of bocaparvovirus in the intestinal tract of tufted deer (Elaphodus cephalophus), tentatively named "Elaphodus cephalophus bocaparvovirus" (ECBOV). A nearly complete genome sequence of 5,354 nucleotides was obtained, which had the typical genome organization and protein motifs of a bocaparvovirus. Sequence comparisons and phylogenetic analysis revealed that ECBOV may be a new ungulate bocaparvovirus. The identification and characterization of viruses in wildlife will facilitate our understanding of genetic evolution and cross-species transmission and thus further reduce the potential threat to human and animal health.
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http://dx.doi.org/10.1007/s00705-021-05308-3 | DOI Listing |
Front Biosci (Elite Ed)
December 2024
Polytechnic School, University of Vale do Itajaí (Univali), Itajaí, SC 88302-202, Brazil.
Background: Enhanced biological phosphorus removal (EBPR) systems utilize phosphorus-accumulating organisms (PAOs) to remove phosphorus from wastewater since excessive phosphorus in water bodies can lead to eutrophication. This study aimed to characterize a newly isolated PAO strain for its potential application in EBPR systems and to screen for additional biotechnological potential. Here, sequencing allowed for genomic analysis, identifying the genes and molecules involved, and exploring other potentials.
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November 2024
Department of Orthopaedics, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, 646000 Luzhou, Sichuan, China.
Background: The mechanism for RNA methylation during disc degeneration is unclear. The aim of this study was to identify N6-methyladenosine (m6A) markers and therapeutic targets for the prevention and treatment of intervertebral disc degeneration (IDD).
Methods: Methylated RNA immunoprecipitation sequencing (MeRIP-seq) and quantitative reverse transcription PCR (RT-qPCR) were employed to analyze m6A modifications of IDD-related gene expression.
J Inflamm Res
December 2024
Department of Dermatology, China-Japan Friendship Hospital, National Center for Integrative Medicine, Beijing, 100029, People's Republic of China.
Background: Psoriasis represents a persistent, immune-driven inflammatory condition affecting the skin, characterized by a lack of well-established biologic treatments without adverse events. Consequently, the identification of novel targets and therapeutic agents remains a pressing priority in the field of psoriasis research.
Methods: We collected single-cell RNA sequencing (scRNA-seq) datasets and inferred T cell differentiation trajectories through pseudotime analysis.
Iran J Parasitol
January 2024
Department of Veterinary Microbiology, University of Ibadan, Ibadan, Nigeria.
Background: Haemonchosis is a major parasitic infestation in ruminant livestock, causing significant economic losses annually. The causative organisms are helminths of the genus spp. Detection of the causative agent is important for effective management and control of the disease.
View Article and Find Full Text PDFFront Plant Sci
December 2024
Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei, China.
Chinese chestnut ( Blume) is an important economic forest tree species and mainly cultivated in mountainous areas and wastelands, subjecting it to various abiotic stresses. The protein phosphatase 2C (PP2C) genes contributes largely to stress responses in plants. However, the characteristics and functions of genes in remain unknown.
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