Galectin-9 Regulates Monosodium Urate Crystal-Induced Gouty Inflammation Through the Modulation of Treg/Th17 Ratio.

Front Immunol

Institute of Cardiovascular Sciences (ICVS), College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.

Published: February 2022

Gout is caused by depositing monosodium urate (MSU) crystals within the articular area. The infiltration of neutrophils and monocytes drives the initial inflammatory response followed by lymphocytes. Interestingly, emerging evidence supports the view that imbalance of T helper 17 cells (Th17)/regulatory T cells (Treg) impacts the subsequent damage to target tissues. Galectin-9 (Gal-9) is a modulator of innate and adaptive immunity with both pro- and anti-inflammatory functions, dependent upon its expression and cellular location. However, the specific cellular and molecular mechanisms by which Gal-9 modulates the inflammatory response in the onset and progression of gouty arthritis has yet to be elucidated. In this study, we sought to comprehensively characterise the functional role of exogenous Gal-9 in an model of MSU crystal-induced gouty inflammation by monitoring neutrophils, monocytes and Th17/Treg recruited phenotypes and related cyto-chemokines profile. Treatment with Gal-9 revealed a dose-dependent reduction in joint inflammation scores, knee joint oedema and expression of different pro-inflammatory cyto-chemokines. Furthermore, flow cytometry analysis highlighted a significant modulation of infiltrating inflammatory monocytes (CD11b/CD115/LY6-C) and Th17 (CD4/IL-17)/Treg (CD4/CD25/FOXP-3) cells following Gal-9 treatment. Collectively the results presented in this study indicate that the administration of Gal-9 could provide a new therapeutic strategy for preventing tissue damage in gouty arthritic inflammation and, possibly, in other inflammatory-based diseases.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581207PMC
http://dx.doi.org/10.3389/fimmu.2021.762016DOI Listing

Publication Analysis

Top Keywords

monosodium urate
8
crystal-induced gouty
8
gouty inflammation
8
neutrophils monocytes
8
inflammatory response
8
gal-9
6
galectin-9 regulates
4
regulates monosodium
4
urate crystal-induced
4
gouty
4

Similar Publications

High-altitude regions are prone to plateau erythrocytosis due to unique geo-climatic conditions such as low oxygen, high altitude, and low temperatures, with significantly higher incidences of hyperlipidemia and hyperuricemia compared to lowland areas. However, the extreme environmental conditions at high altitudes and the elevated hematocrit levels in blood samples from these populations present significant challenges to the applicability of existing point-of-care testing (POCT) devices. This study describes the development, early clinical validation, and potential clinical impact of three portable, paper-based photochemical biosensing platforms specifically designed for use at high altitudes to monitor hemoglobin, lipids, and uric acid in blood samples.

View Article and Find Full Text PDF

Tinospora crispa (L.) Hook.f. & Thomson vines ameliorates hyperuricemia by inhibiting synthesis and promoting excretion of uric acid through targeting NLRP3/caspase-1/IL-1β pathway.

J Ethnopharmacol

December 2024

Department of Rheumatology and Immunology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, 510280, China; School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong, 510515, China; Guangdong Province Key Laboratory of Immune Regulation and Immunotherapy, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong, 510515, China. Electronic address:

Ethnopharmacological Relevance: Tinospora crispa (L.) Hook.f.

View Article and Find Full Text PDF

NAD deficiency plays essential roles in the hyperuricemia of stroke-prone spontaneously hypertensive rat via xanthine dehydrogenase to xanthine oxidase conversion.

Biochem Biophys Res Commun

December 2024

Department of Internal Medicine 1, Shimane University Faculty of Medicine, 89-1 Enya-cho, Izumo, Shimane, 693-8501, Japan; The Center for Integrated Kidney Research and Advance, Faculty of Medicine, Shimane University, 89-1 Enya-cho, Izumo, 693-8501, Japan. Electronic address:

Inhibition of xanthine oxidoreductase (XOR) was shown to ameliorate the stroke susceptibility in the stroke-prone spontaneously hypertensive rat (SHRSP), suggesting hyperuricemia had a pathological role in this rat model. In this study, we thus aimed to explore mechanisms inducing hyperuricemia in SHRSP. XOR is known to have two forms, xanthine dehydrogenase (XDH) as the prototype and xanthine oxidase (XO) as the converted form through cleavage of a peptide bond or through formation of disulfide bonds in the enzyme.

View Article and Find Full Text PDF

Fe/Pt-doped carbon nanoparticles with peroxidase-like activity for point-of-care determination of uric acid.

Mikrochim Acta

December 2024

Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, 030032, China.

A pasting-3D microfluidic paper-based analytical device (P-3D μPAD) was developed. It enabled an efficient cascade reaction between urate oxidase (UOX) and Fe/Pt-doped carbon nanoparticles (Fe/Pt-CNPs) for visual colorimetric detection of uric acid (UA). The novel Fe/Pt-CNP nanozyme performed high peroxidase-like activity toward 3,3',5,5'-tetramethylbenzidine (TMB) and HO with Michaelis - Menten constants (K) of 0.

View Article and Find Full Text PDF

Application of single venous approach under echocardiography without angiography in closure of Patent Ductus Arteriosus.

J Cardiothorac Surg

December 2024

Department of Cardiothoracic Surgery, Suining Central Hospital, No. 127, Desheng Road, Chuanshan District, Suining, 629000, China.

Background: The conventional arteriovenous approach closure of patent ductus arteriosus (PDA) may be associated with more complications, especially in young infants. The objective is to explore the feasibility and clinical efficacy of interventional closure of PDA through a single venous approach under echocardiography without angiography.

Methods: 112 patients (32 males and 80 females) with PDA closed by different methods in Suining Central Hospital were enrolled, including 60 cases (Group 1) with a single venous approach under echocardiography without angiography and 52 cases (Group 2) with the conventional arteriovenous approach.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!