Collagen XII is a regulator of corneal stroma structure and function. The current study examined the role of collagen XII in regulating corneal stromal transforming growth factor (TGF)-β activation and latency. Specifically, with the use of conventional collagen XII null mouse model, the role of collagen XII in the regulation of TGF-β latency and activity in vivo was investigated. Functional quantification of latent TGF-β in stromal matrix was performed by using transformed mink lung reporter cells that produce luciferase as a function of active TGF-β. Col12a1 knockdown with shRNA was used to test the role of collagen XII in TGF-β activation. Col12a1 hypertrophic stromata were observed with keratocyte hyperplasia. Increased collagen fibril forward signal was found by second harmonic generation microscopy in the absence of collagen XII. Collagen XII regulated mRNA synthesis of Serpine1, Col1a1, and Col5a1 and deposition of collagens in the extracellular matrix. A functional plasminogen activator inhibitor luciferase assay showed that collagen XII is necessary for latent TGF-β storage in the extracellular matrix and that collagen XII down-regulates active TGF-β. Collagen XII dictates stromal structure and function by regulating TGF-β activity. A hypertrophic phenotype in Col12a1 corneal tissue can be explained by abnormal up-regulation of TGF-β activation and decreased latent storage.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908044 | PMC |
| http://dx.doi.org/10.1016/j.ajpath.2021.10.014 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative transcriptome analysis of albino northern snakehead (Channa argus) reveals its various collagen-related DEGs in caudal fin cells.
PLoS One
December 2024
College of Life Sciences, Neijiang Normal University, Neijiang, China.
The albino northern snakehead (Channa argus) is an aquaculture species characterized by heritable albino body color, in contrast to the typical coloration. Additionally, there are gray- and golden-finned individuals, which exhibit distinct coloration in their caudal fins. We performed RNA-seq to profile the transcriptome of caudal fin tissues in albino gray-finned and golden-finned C.
View Article and Find Full Text PDFEffects of Nanofat and PRP on Type I Collagen Production in Striae Distensae: Preliminary Findings from a Prospective, Randomized Single-Blind Study.
Aesthetic Plast Surg
December 2024
Department of Plastic, Reconstructive and Maxillo facial Surgery, Henri Mondor Hospital, University Paris XII, 51 Avenue du Maréchal de Lattre de Tassigny, 94000, Créteil, France.
Introduction: Striae distensae (SD) appear clinically as parallel striae, lying perpendicular to the tension lines of the skin. SD evolve into two clinical phases, an initial inflammatory phase in which they are called "striae rubrae" (SR) and a chronic phase in which they are called striae albae (SA). This study investigates the synergistic effect of nanofat and platelet-rich plasma (PRP) injections on collagen production in fibroblasts derived from SA (SAF).
View Article and Find Full Text PDFA single-domain antibody targeting factor XII inhibits both thrombosis and inflammation.
Nat Commun
September 2024
State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Article Synopsis
- Factor XII (FXII) is crucial for activating the body's intrinsic coagulation pathway and has a previously unclear role in inflammation.
- Treating male mice with a novel antibody (Nb-Fc) that targets FXII significantly reduced arterial thrombosis without disrupting normal blood clotting.
- The study shows that inhibiting FXII can lower inflammation-related symptoms and complications during procedures like extracorporeal membrane oxygenation (ECMO), indicating its potential as a therapeutic target for thrombo-inflammatory diseases.
Transcriptome analysis reveals the pathogenesis of spontaneous tibial dyschondroplasia in broilers.
Front Genet
July 2024
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Tibial dyschondroplasia (TD) is a severe bone disease that affects fast-growing broiler chickens and causes economic loss. Despite previous studies, the regulatory mechanism of TD remains unclear and is thought to be primarily based on thiram induction, which may differ from that of naturally occurring diseases. To better understand TD, a digital X-ray machine was used in the present study to determine its incidence in four hundred yellow-feathered broiler chickens.
View Article and Find Full Text PDFType XII collagen is elevated in serum from patients with solid tumors: a non-invasive biomarker of activated fibroblasts.
Clin Exp Med
July 2024
Nordic Bioscience A/S, 2730, Herlev, Denmark.
Understanding the tumor microenvironment (TME) and extracellular matrix (ECM) is crucial in cancer research due to their impact on tumor progression. Collagens, major ECM components, regulate cell signaling and behavior. Of the 28 reported collagens, type XII collagen is known to be vital for ECM organization.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!