Double and triple bonds have significant effects on the biological activities of lipids. Determining multiple bond positions in their molecules by mass spectrometry usually requires chemical derivatization. This work presents an HPLC/MS method for pinpointing the double and triple bonds in fatty acids. Fatty acid methyl esters were separated by reversed-phase HPLC with an acetonitrile mobile phase. In the APCI source, acetonitrile formed reactive species, which added to double and triple bonds to form [M + CHN] ions. Their collisional activation in an ion trap provided fragments helpful in localizing the multiple bond positions. This approach was applied to fatty acids with isolated, cumulated, and conjugated double bonds and triple bonds. The fatty acids were isolated from the fat body of early-nesting bumblebee and seeds or seed oils of , , and . Using the method, the presence of the known fatty acids was confirmed, and new ones were discovered.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588306 | PMC |
| http://dx.doi.org/10.3390/molecules26216468 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chlamydomonas reinhardtii polysaccharides retard rice starch retrogradation by weakening hydrogen bond strength within starch double helices.
Int J Biol Macromol
January 2025
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address:
This study investigated the effects of Chlamydomonas reinhardtii polysaccharides (CRPs) on retarding the retrogradation of japonica rice starch (JS) and glutinous rice starch (GS). Structure characterization revealed that CRPs, with an average molecular weight of 505 kDa, mainly consisted of glucose, mannose, and galactose and featured a triple-helix structure. CRPs could reduce the storage modulus increment of JS during the cooling process by interacting with amylose, thereby inhibiting gel network formation.
View Article and Find Full Text PDFNonconventional Full-Color Luminescent Polyurethanes: Luminescence Mechanism at the Molecular Orbital Level.
ACS Mater Lett
January 2025
Department of Chemistry, Durham University, Durham, DH1 3LE, U.K.
The study of structure-activity relationships is a top priority in the development of nontraditional luminescent materials. In this work, nonconjugated polyurethanes (PUs) with full-color emission (red, green, and blue) are easily obtained by control of the diol monomer structure and the polymerization conditions. Selected diol monomers introduced single, double, or triple bond repeating units into the main chain of the PUs, in order to understand how unsaturated bonds and H-bonds affect their luminescence from a molecular orbital viewpoint.
View Article and Find Full Text PDFChemical Bonding and Dynamic Structural Fluxionality of a Boron-Based BAl Cluster.
Molecules
December 2024
Department of Chemical and Materials Engineering, Lyuliang University, Lishi 033001, China.
We studied the boron-based composite cluster BAl doped with Al atoms. The global minimum structure of the BAl cluster is a three-layer structure, consisting of three parts: an Al unit, a B ring and an isolated Al atom. Charge calculations analysis shows that the cluster can be expressed as [Al][B][Al], has 6π/6σ double aromaticity and follows the (4+2) Hückel rule.
View Article and Find Full Text PDFInvestigation on the Coordination Bonding Nature of Actinide-Doped Endohedral Borospherenes An@B (An = U, Np, Pu, Am, Cm).
Molecules
December 2024
Key Laboratory of Chemical Biology and Molecular Engineering of Education Ministry, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Endohedral metallo-borospherenes M@B have received considerable attention since the discovery of B in 2014. However, the coordination bonding nature of most of actinide-doped endohedral An@B still remains in dispute or unexplored. Extensive and systematic first-principles theory calculations performed herein unveil the ground states of triplet U@B (, , A), quartet U@B (, , B), quintet Np@B (, , A), sextet Np@B (, , A), septet Pu@B (, , A), octet Am@B (, , A), and octet Cm@B (, , A) at the coupled-cluster with triple excitations CCSD(T) level.
View Article and Find Full Text PDFCharacterization and film-forming properties of collagen from three species of sea cucumber from the South China Sea: Emphasizing the effect of transglutaminase.
Int J Biol Macromol
January 2025
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, China; National Research and Development Branch Center for Shellfish Processing (Zhanjiang), Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524088, China. Electronic address:
This study aimed to investigate the structural characteristics of Stichopus horrens collagen (SHC), Holothuria scabra collagen (HSC), and Holothuria leucospilota collagen (HLC) and to assess the effect of transglutaminase (TGase) on their film-forming properties. The results indicated that the collagens from three species of sea cucumbers were type I collagen with a complete triple helical structure. The thermal denaturation temperature of HLC (34.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!