AI Article Synopsis
- Nitric oxide (NO) is crucial for immune defense against pathogens, but its absence in inducible nitric oxide synthase knockout Sprague Dawley rats shows these rats can resist infection entirely.
- Infection in rat peritoneal macrophages leads to high reactive oxygen species (ROS) levels due to GRA43, which damages cell membranes and triggers cell death.
- The study highlights the relationship between NO and ROS in immunity, suggesting that the -SD rat may possess an alternative innate immune system against infections.
Article Abstract
Nitric oxide (NO) is an important immune molecule that acts against extracellular and intracellular pathogens in most hosts. However, after the knockout of inducible nitric oxide synthase ( ) in Sprague Dawley (SD) rats, these rats were found to be completely resistant to infection. Once the rat peritoneal macrophages (PMs) were infected with , they produced high levels of reactive oxygen species (ROS) triggered by GRA43 secreted by , which damaged the parasitophorous vacuole membrane and PM mitochondrial membranes within a few hours post-infection. Further evidence indicated that the high levels of ROS caused mitochondrial superoxide dismutase 2 depletion and induced PM pyroptosis and cell death. This discovery of complete resistance to infection, in the -SD rat, demonstrates a strong link between NO and ROS in immunity to infection and showcases a potentially novel and effective backup innate immunity system.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571494 | PMC |
| http://dx.doi.org/10.1016/j.isci.2021.103280 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!