AI Article Synopsis
- This study investigates how TGF-β1-overexpressed mesenchymal stromal cells (MSCs) can suppress T cell activity, which is important for organ transplants.
- Mesenchymal stromal cells were isolated from canine bone marrow, modified to overexpress TGF-β1 using lentivirus, and then co-cultured with T cells to observe their effects on immune regulation.
- Findings revealed that TGF-β1/MSCs reduced the amount of certain T cells while increasing regulatory T cells and altering the expression of key cytokines, indicating a robust immunosuppressive role possibly mediated by interferon-γ.
Article Abstract
Transforming growth factor (TGF)-β1 and mesenchymal stromal cells (MSCs) are two effective immunosuppressive agents for organ transplantation technology. This study aims to explore the molecular mechanism of TGF-β1-overexpressed MSCs on T cell immunosuppression. To achieve that, BM-MSCs were isolated from canine bone marrow, and their osteogenic differentiation and surface markers were detected. The TGF-β1 gene was transferred into lentivirus and modified MSCs (TGF-β1/MSCs) by lentivirus transfection. Furthermore, TGF-β1/MSCs were co-cultured with T cells to investigate their effect on differentiation and immune regulation. Results showed that TGF-β1/MSCs significantly downregulated the proportion of CD4 CD8 T cells in lymphocytes and significantly upregulated the proportion of CD4 CD25 T cells. Moreover, TGF-β1/MSCs significantly upregulated the expression of IL-10 in CD4 T cells and downregulated the expression of IL-17A, IL-21, and IL-22. Meanwhile, interferon-γ (IFN-γ) neutralizing antibody blocked the effects of TGF-β1/MSCs on the differentiation inhibition of Th17. Overall, our results confirm the strong immunosuppressive effect of TGF-β1/MSCs and demonstrate that IFN-γ mediates the immunosuppressive effect of TGF-β1/MSC.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565592 | PMC |
| http://dx.doi.org/10.1515/biol-2021-0118 | DOI Listing |
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