Background And Objectives: To define the clinical and pathologic correlations of compartmentalized perivascular B cells in postmortem progressive multiple sclerosis (MS) brains.
Methods: Brain slices were acquired from 11 people with secondary progressive (SP) MS, 5 people with primary progressive (PP) MS, and 4 controls. Brain slices were immunostained for B lymphocytes (CD20), T lymphocytes (CD3), cytotoxic T lymphocytes (CD8), neuronal neurofilaments (NF200), myelin (SMI94), macrophages/microglia (CD68 and IBA1), astrocytes (glial fibrillary acidic protein [GFAP]), and mitochondria (voltage-dependent anion channel and cytochrome c oxidase subunit 4). Differences in CD20 immunostaining intensity between disease groups and associations between CD20 immunostaining intensity and both clinical variables and other immunostaining intensities were explored with linear mixed regression models and Cox regression models, as appropriate.
Results: CD20 immunostaining intensity was higher in PPMS (Coeff = 0.410; 95% confidence interval [CI] = 0.046, 0.774; = 0.027) and SPMS (Coeff = 0.302; 95% CI = 0.020, 0.585; = 0.036) compared with controls. CD20 immunostaining intensity was higher in cerebellar, spinal cord, and pyramidal onset (Coeff = 0.274; 95% CI = 0.039, 0.510; = 0.022) compared with optic neuritis and sensory onset. Higher CD20 immunostaining intensity was associated with younger age at onset (hazard ratio [HR] = 1.033; 95% CI = 1.013, 1.053; = 0.001), SP conversion (HR = 1.056; 95% CI = 1.022, 1.091; = 0.001), wheelchair dependence (HR = 1.472; 95% CI = 1.108, 1.954; = 0.008), and death (HR = 1.684; 95% CI = 1.238, 2.291; = 0.001). Higher immunostaining intensity for CD20 was associated with higher immunostaining intensity for CD3 (Coeff = 0.114; 95% CI = 0.005, 0.224; = 0.040), CD8 (Coeff = 0.275; 95% CI = 0.200, 0.350; < 0.001), CD68 (Coeff = 0.084; 95% CI = 0.023, 0.144; = 0.006), GFAP (Coeff = 0.002; 95% CI = 0.001, 0.004; = 0.030), and damaged mitochondria (Coeff = 3.902; 95% CI = 0.891, 6.914; = 0.011).
Discussion: Perivascular B cells were associated with worse clinical outcomes and CNS-compartmentalized inflammation. Our findings further support the concept of targeting compartmentalized B-cell inflammation in progressive MS.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587731 | PMC |
http://dx.doi.org/10.1212/NXI.0000000000001108 | DOI Listing |
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