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http://dx.doi.org/10.1111/apa.16180 | DOI Listing |
Chembiochem
March 2025
Sirius university of Science and Technology, Research Center for Translational Medicine, Olympic Ave., 1, 354340, Sochi, RUSSIAN FEDERATION.
Fluorescent protein-based biosensors are indispensable molecular tools in cell biology and biomedical research, providing non-invasive dynamic measurements of metabolite concentrations and other cellular signals. Traditional methods for developing these biosensors rely on rational design, but directed evolution methods offer a more efficient alternative. This review discusses recent advancements in the development of biosensors using directed evolution, including methods for optimizing domain fusions, sequence optimization, and new screening and selection systems.
View Article and Find Full Text PDFJ Forensic Sci
March 2025
Department of Forensic Science, College of Criminal Justice, Sam Houston State University, Huntsville, Texas, USA.
Ayahuasca contains N,N-dimethyltryptamine (DMT), the primary alkaloid responsible for its psychedelic effects. DMT oxidative deamination yields indole-3-acetic acid (IAA) as the predominant metabolite, while N-oxidation produces N,N-dimethyltryptamine-N-oxide (DMT-NO) as the second most abundant metabolite. An LC-MS/MS method was developed and validated to quantify DMT, IAA, and DMT-NO in human plasma, as well as DMT and DMT-NO in human urine.
View Article and Find Full Text PDFAims: Inter-atrial shunt device therapy has shown mixed results in clinical trials, with clinical 'non-responders' typically showing features of more advanced heart failure. We aimed to analyse the haemodynamic and clinical response of a novel left atrial to coronary sinus (LA-CS) shunt device in patients with higher natriuretic peptide (NP) levels, a marker of disease severity.
Methods And Results: An analysis population (n = 95) of patients from the ALT-FLOW early feasibility study with left ventricular ejection fraction >40% and 1-year follow up was analysed.
Psychooncology
March 2025
Faculty of Social Welfare and Health Sciences, School of Social Work, University of Haifa, Haifa, Israel.
Background: Research on the attitudes and support received by cancer survivors with preexisting severe mental health conditions (SMHC) from their families and oncology professionals is lacking.
Aims: To explore how individuals with SMHC perceive and experience family and oncology team attitudes and care.
Methods: Participants were 25 cancer survivors, 6 men and 18 women, aged 26-86 with preexisting SMHC.
Ann Ital Chir
March 2025
Operative Research Unit of General Surgery, Fondazione Policlinico Universitario Campus Bio-Medico, 00128 Rome, Italy; Research Unit of General Surgery, Università Campus Bio-Medico di Roma, 00128 Rome, Italy.
No abstract present.
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