This study evaluated the stability of casein phosphopeptides (CPP) and obtained peptide-calcium complex by heating and chelating the peptide with CaCl in a neutral solution. To assess the bioavailability of various calcium formulations, the calcium transport models were established in Caco-2 cells, and the transcellular transport pathways of various calcium formulations were studied by RT-PCR and Western blotting. Results of circular dichroism showed that CPP was a stable polypeptide. The ultraviolet absorption spectrum and Fourier transform-infrared spectrum (FT-IR) indicated that calcium could be chelated by carboxyl oxygen and amino nitrogen atoms of CPP to form peptide calcium chelate, and the calcium bioavailability of peptide calcium chelate was significantly higher than that of CaCl , calcium l-aspartate, and casein phosphopeptides mixed with CaCl . Four calcium sources increased the expression of TRPV5 and TRPV6 genes and proteins. The study intended to provide a basis for developing a novel calcium supplement. PRACTICAL APPLICATIONS: This paper examined the bioavailability of casein phosphopeptides calcium complex, CaCl , calcium l-aspartate, and casein phosphopeptides mixed with CaCl in Caco-2 cells, and the mechanisms were detected by western blotting. The results provide theoretical knowledge for the selection of calcium supplement raw materials and lay a foundation for the development of compound calcium preparations and drugs in the future.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/jfbc.14001 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of Gluma® and MI Varnish™ for In-Office Treatment of Dentinal Hypersensitivity: An Observational Study.
Cureus
December 2024
Department of Periodontology and Implantology, Government Dental College and Hospital, Jamnagar, Jamnagar, IND.
Introduction In their routine practice, dentists frequently encounter dentinal hypersensitivity, which is caused by the pulpal nerves' increased excitability due to fluid movement in the dentinal tubules. It is treated in-office using dentin desensitizers, which reduce hypersensitivity by obstructing the open tubules or desensitizing the free nerve endings present within the tubules. However, no substance or treatment plan has ever been proven to be the gold standard for the efficient treatment of dentinal hypersensitivity.
View Article and Find Full Text PDFReview: Application of Protein-Based Raw Materials in Health Foods in China.
Foods
December 2024
College of Biochemical Engineering, Beijing Union University, Beijing 100023, China.
Raw protein materials are beneficial for human health, so they are being increasingly used in health foods. In recent years, there has been more and more research on and applications of raw protein materials, but few teams have conducted a detailed review of the application status of raw protein materials in China's health foods, the basis for their compliance and use, and the research on their health care functions. Therefore, this review evaluates the application of animal and plant proteins in China's health foods, the impact of animal and plant proteins on human health, and future research recommendations for animal and plant proteins.
View Article and Find Full Text PDFAssessment of Change in Enamel Color and Surface Hardness Following the Use of ICON Resin Infiltration and Remineralizing Agent: An In Vitro Study.
Materials (Basel)
December 2024
Independent Researcher, P.O. Box 60169-38, Riyadh 11545, Saudi Arabia.
This study aimed to evaluate the change in enamel color and surface micro-hardness following the use of resin-infiltration concept material (ICON) and casein phosphopeptide-amorphous calcium fluoride phosphate (CPP-ACFP) remineralizing agent. Fifty-four extracted human third molars were collected and randomly divided into three groups: group A: control with no surface treatment; group B: treated using ICON; and group C: treated using CPP-ACFP. The change in color and micro-hardness of the enamel surface were measured using spectrophotometer and Vickers hardness number, respectively.
View Article and Find Full Text PDFComparative analysis of the effect of self-assembling peptide P-4 on enamel erosion: a confocal laser scanning microscopy study.
Clin Oral Investig
December 2024
Department of Pedodontics, Faculty of Dentistry, Izmir Katip Celebi University, Izmir, Turkey.
Objectives: This study aims to comparatively assess the preventive and protective effects of the self-assembling peptide P-4 on enamel erosion and evaluate the potential for enamel surface recovery when professional products are combined with home-use dental-care products during the erosive process.
Materials And Methods: Ninety-nine bovine incisors were divided into nine groups: a control group, four groups with the application of professional-products [P-4 peptide (Curodont-Repair), stannous/Sn containing solution (8% Sn), casein-phosphopeptide-amorphous-calcium-phosphate fluoride/CPP-ACPF (MI Varnish), sodium fluoride/NaF (Profluorid)] and four groups with the combination of professional products and home-use daily dental care products [P-4 peptide (Curodont Repair + Curodont Protect), stannous ions containing agents (8% Sn+Emofluor Gel Intensive-Care), CPP-ACPF (MI Varnish + MI Paste Plus), NaF (Profluorid + ReminPro)]. Professional products were applied once before a five-day erosive cycle, involving six 2-minute citric-acid exposures per day.
Non-Invasive Strategies for Remineralization and Hypersensitivity Management in Molar-Incisor Hypomineralization-A Systematic Review and Meta-Analysis.
J Clin Med
November 2024
Centre for Translational Medicine, Semmelweis University, 1085 Budapest, Hungary.
Molar-incisor hypomineralization (MIH) is an enamel defect affecting molars and incisors, often leading to hypersensitivity, enamel breakdown, and increased caries risk. Non-invasive treatments, such as casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride varnish, show potential in remineralizing affected enamel and reducing sensitivity, but their efficacy is still debated. This study systematically reviews and analyzes the effectiveness of CPP-ACP and other non-invasive agents in improving remineralization and reducing hypersensitivity in MIH-affected teeth.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!