A greenhouse experiment aimed to assess the effects of poultry manure, sorghum, and clover residues (0 and 15 g kg) on the zinc (Zn) bioavailable fraction in contaminated calcareous soil using two chemical assays, including diffusion gradient in thin-films (DGT) and diethylene triamine pentaacetic acid-triethanolamine (DTPA-TEA), and a bioassay with corn (Zea mase L.). The results showed that poultry manure, clover, and sorghum residues application increased dissolved organic carbon (DOC) by 53.6 and 36.1, and 9.2%, respectively, and decreased soil pH by 0.42, 0.26, and 0.06 units, respectively compared to unamended soil. These changes resulted in increases of Zn effective concentration (C) and DTPA-Zn, and plant Zn concentration as observed by the increase in exchangeable form of Zn. In the sorghum residues-amended soils, C-Zn decreased by 29.5% compared to other treatments. The best correlations between corn metal concentrations and soil metal bioavailability were obtained for C-Zn using the DGT technique, which also provided the best Zn bioavailability estimate. It is concluded that sorghum residues could be used to reduce the phytotoxicity risk of Zn in calcareous contaminated soil, and the DTPA method is the less robust indicator of Zn bioavailability than the DGT technique.
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