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model of bacterial biofilm mineralization in complex humid environments: a proof of concept study.
Front Bioeng Biotechnol
January 2025
Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
Background: Bacteria in physiological environments can generate mineralizing biofilms, which are associated with diseases like periodontitis or kidney stones. Modelling complex environments presents a challenge for the study of mineralization in biofilms. Here, we developed an experimental setup which could be applied to study the fundamental principles behind biofilm mineralization on rigid substrates, using a model organism and in a tailored bioreactor that mimics a humid environment.
View Article and Find Full Text PDFLunate prosthetic arthroplasty for the treatment of Kienbock's disease: A systematic review.
J Hand Microsurg
March 2025
Pulvertaft Hand Centre, Royal Derby Hospital, United Kingdom.
Purpose: Prosthetic replacement of the fragmented, collapsed and unreconstructable lunate is a treatment option in Kienbock's disease. The objective of this study is to assess the effectiveness, safety and outcomes of prosthetic lunate arthroplasty for the treatment of Kienbock's disease.
Methods: We conducted a PRISMA-P compliant systematic review PROSPERO (CRD 42021283996) searching Cochrane, Medline, PubMed and Embase databases for the outcomes of prosthetic lunate arthroplasty.
A novel method for expressing and purifying large quantities of functional and stable human voltage-gated proton channel (hH1).
Protein Sci
February 2025
Cell Physiology and Molecular Biophysics Department, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.
Purifying membrane proteins has been the limiting step for studying their structure and function. The challenges of the process include the low expression levels in heterologous systems and the requirement for their biochemical stabilization in solution. The human voltage-gated proton channel (hH1) is a good example of that: the published protocols to express and purify hH1 produce low protein quantities at high costs, which is an issue for systematically characterizing its structure and function.
View Article and Find Full Text PDFEvaluation of a novel modified selective medium cefixime-tellurite-phosphate-xylose-rhamnose MacConkey agar for the isolation of Escherichia albertii from diarrheal stool specimens.
J Vet Med Sci
January 2025
Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
It is challenging to isolate Escherichia albertii from clinical specimens. Therefore, a medium that can selectively grow E. albertii and differentiate it from E.
View Article and Find Full Text PDFOccupational management of low back pain secondary to prolapsed intervertebral disc in a Malaysian healthcare worker: a case report emphasizing ergonomic intervention and risk reduction strategies.
Ind Health
January 2025
Department of Social and Preventive Medicine, Faculty of Medicine, Universiti Malaya, Malaysia.
Low back pain (LBP) is a commonly encountered medical disorder in Malaysia's primary care setting, though establishing a direct connection between LBP and the workplace environment in adults is challenging. This case presents a clinic nurse who developed LBP due to a prolapsed intervertebral disc and her clinical management from an Occupational Health Doctor perspective. Her occupational management involved a walk-through survey at an urban hospital, which identified bone marrow aspiration as her most physically demanding task.
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