AI Article Synopsis

  • Transovarial transmission (TOT) of the dengue virus (DENV) in Aedes mosquitoes is crucial for the virus's survival in nature and potentially for triggering outbreaks.
  • The study focused on detecting TOT in wild populations of Aedes albopictus in Cuba, collecting mosquito larvae from Cotorro municipality.
  • Out of 15 larval groups tested, 4 tested positive for DENV-3, marking the first evidence of TOT in field populations of Aedes albopictus in Cuba and highlighting its potential role as a DENV vector.

Article Abstract

Transovarial transmission (TOT) of dengue virus (DENV) in Aedes spp. is an important mechanism for DENV maintenance in nature and may be important in initiating outbreaks. The objective of this study was to explore the occurrence of TOT in wild Aedes albopictus populations in Cuba. Mosquito larvae were collected in Cotorro municipality, Havana, Cuba, and identified to species. Fifteen pools of Ae. albopictus each containing 30 larvae were processed for DENV detection by using conventional reverse transcription polymerase chain reaction (RT-PCR) and nested PCR. Four out of 15 pools processed were positive for DENV-3, but no other DENV serotype was detected. This is the first time TOT of DENV detected in Cuban field populations of Ae. albopictus, and this suggests that this species may be an important vector of DENV in Cuba.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8832932PMC
http://dx.doi.org/10.4269/ajtmh.21-0710DOI Listing

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