Transovarial transmission (TOT) of dengue virus (DENV) in Aedes spp. is an important mechanism for DENV maintenance in nature and may be important in initiating outbreaks. The objective of this study was to explore the occurrence of TOT in wild Aedes albopictus populations in Cuba. Mosquito larvae were collected in Cotorro municipality, Havana, Cuba, and identified to species. Fifteen pools of Ae. albopictus each containing 30 larvae were processed for DENV detection by using conventional reverse transcription polymerase chain reaction (RT-PCR) and nested PCR. Four out of 15 pools processed were positive for DENV-3, but no other DENV serotype was detected. This is the first time TOT of DENV detected in Cuban field populations of Ae. albopictus, and this suggests that this species may be an important vector of DENV in Cuba.
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http://dx.doi.org/10.4269/ajtmh.21-0710 | DOI Listing |
Insects
December 2024
School of Integrative Plant Science, Plant Pathology and Plant-Microbe Biology, Cornell University, Geneva, NY 14456, USA.
Grapevine red blotch is an emerging disease that threatens vineyard productions in North America. Grapevine red blotch virus (GRBV, species , genus , family ), the causal agent of red blotch disease, is transmitted by (Hemiptera: Membracidae) in a circulative, non-propagative mode. To gain new insight into GRBV- interactions, we delved into vertical transmission and documented a lack of transovarial transmission.
View Article and Find Full Text PDFVet Parasitol
December 2024
Instituto de Investigación de la Cadena Láctea (IdICaL) (INTA, CONICET), Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Ruta 34 km 227, Rafaela, Santa Fe CP 2300, Argentina.
This study aimed to evaluate A. marginale transstadial (TST) and transovarial transmission (TOT) by Amblyomma tonelliae through vector competence assays and analysis of natural infection in free-living ticks. This three-host tick species was chosen as model because it is a usual parasite of cattle in all their parasitic stages, making them potential vectors through TST or TOT.
View Article and Find Full Text PDFExp Appl Acarol
December 2024
Zoological Institute of Russian Academy of Sciences, Universitetskaya embankment, 1, St. Petersburg, 199034, Russia.
In this study, molecular analysis was used to show the possibility of transovarial and transstadial transmission of Borrelia valaisiana in Ixodes ricinus ticks in the natural biotopes of North-Western Russia. Female ticks collected in nature were fed on rabbits; larvae obtained from these females were fed on white mice. Eggs, female ticks after the egg deposition, larvae and nymphs of ticks after overwintering were studied for Borrelia burgdorferi s.
View Article and Find Full Text PDFTicks Tick Borne Dis
November 2024
Clinic of Internal Medicine with the Occupational Diseases and the Diagnostics Subunit, Institute of Rural Health, Lublin, Poland. Electronic address:
Research on the transovarial transmission of pathogens whose reservoirs and vectors are ticks has led to an understanding of the mechanisms related to the circulation and persistence of selected microorganisms in natural foci. The primary aim of this study was to investigate the possibility of transovarial transmission of Rickettsia spp. in Dermacentor reticulatus ticks, and the influence of Francisella-like endosymbionts (FLEs) and Spiroplasma spp.
View Article and Find Full Text PDFFront Cell Infect Microbiol
November 2024
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig, Türkiye.
, commonly associated with ovine babesiosis, poses a significant threat to sheep health, often resulting in severe clinical manifestations and high mortality rates. However, the impact of on goats has remained uncertain, prompting us to investigate its pathogenicity in caprine hosts. Experimental infections using -infected blood inoculation and infected tick infestation, were conducted on spleen-intact (n=5) and splenectomized (n=5) goats.
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