Oral mucosal tissue is composed of several cell types that are difficult to dissociate while maintaining high cell viability. We describe a protocol for the preparation and dissociation of human buccal and gingival oral mucosal tissue to a high-viability single-cell suspension composed of heterogeneous cell types. This heterogeneous cell suspension can subsequently be used for cytometric analyses or to generate single-cell RNA sequencing libraries. For complete details on the use and execution of this protocol, please refer to Williams et al. (2021).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551218 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.100908 | DOI Listing |
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