Synthetic biology has been developing rapidly in the last decade and is attracting increasing attention from many plant biologists. The production of high-value plant-specific secondary metabolites is, however, limited mostly to microbes. This is potentially problematic because of incorrect post-translational modification of proteins and differences in protein micro-compartmentalization, substrate availability, chaperone availability, product toxicity, and cytochrome p450 reductase enzymes. Unlike other heterologous systems, plant cells may be a promising alternative for the production of high-value metabolites. Several commercial plant suspension cell cultures from different plant species have been used successfully to produce valuable metabolites in a safe, low cost, and environmentally friendly manner. However, few metabolites are currently being biosynthesized using plant platforms, with the exception of the natural pigment anthocyanin. Both and cell cultures can be developed by multiple gene transformations and CRISPR-Cas9 genome editing. Given that the introduction of heterologous biosynthetic pathways into and is not widely used, the biosynthesis of foreign metabolites is currently limited; however, therein lies great potential. Here, we discuss the exemplary use of plant cell cultures and prospects for using and cell cultures to produce valuable plant-specific metabolites.
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http://dx.doi.org/10.1016/j.xplc.2021.100235 | DOI Listing |
Sci Rep
January 2025
Microbial Safety Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, Republic of Korea.
The close genetic resemblance between Listeria monocytogenes and Listeria innocua, combined with their presence in similar environments, poses challenges for species-specific detection in food products. Ensuring food safety through microbiological standards necessitates reliable detection of pathogens like L. monocytogenes and L.
View Article and Find Full Text PDFMed Mycol
January 2025
Division of Infectious Diseases, Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
Given extensive improvements in access to antiretroviral therapy (ART) over the past 12 years, the HIV and cryptococcal meningitis landscapes have dramatically changed since 2010. We sought to evaluate changes in clinical presentation and clinical outcomes of people presenting with HIV-associated cryptococcal meningitis between 2010 and 2022 in Uganda. We analyzed three prospective cohorts of HIV-infected Ugandans with cryptococcal meningitis during 2010-2012, 2013-2017, and 2018-2022.
View Article and Find Full Text PDFMetab Eng
January 2025
Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA. Electronic address:
Amino acid auxotrophy refers to an organism's inability to synthesize one or more amino acids that are required for cell growth. In microbiome research, co-cultures of amino acid auxotrophs are often used to investigate metabolite cross-feeding interactions and model community dynamics. Thus far, it has been implicitly assumed that amino acids are mainly cross-fed between these auxotrophs.
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January 2025
Plant Morphogenesis and Biochemistry Laboratory, Federal University of Santa Catarina, 88049-900, P. O. Box 476, Florianopolis, SC, Brazil. Electronic address:
The effect of the in vitro acute exposure to diesel oil (0.001%, 0.01%, 0.
View Article and Find Full Text PDFCurr Opin Biotechnol
January 2025
Department of Plant and Microbial Biology and NC Plant Sciences Initiative, North Carolina State University, Raleigh, NC 27695, USA. Electronic address:
The integration of 3D bioprinting into plant science and biotechnology is revolutionizing research and applications. While many high-throughput techniques have advanced plant biology, replicating the complex 3D organization and cellular environments of plant tissues remains a significant challenge. Traditional 2D culture systems fall short of capturing the necessary spatial context for accurate studies of cell behavior, gene expression, and tissue development.
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