AI Article Synopsis
- Spheroid cultures of primary human hepatocytes (PHH) are crucial for studying liver drug metabolism and toxicity, but varying culture conditions can lead to inconsistent results.
- The study examined how different media compositions impact spheroid formation, protein expression, and functionality over three weeks, identifying that certain conditions closely mimic normal PHH characteristics.
- Results indicate that media with specific glucose and insulin levels yield the healthiest PHH spheroids, while more expensive formulations led to significant deviations from native PHH, providing a reliable protocol for hepatocyte research.
Article Abstract
Spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. The cultures are maintained under different conditions, with possible confounding results. We performed an in-depth analysis of the influence of various culture conditions to find the optimal conditions for the maintenance of an like phenotype. The formation, protein expression, and function of PHH spheroids were followed for three weeks in a high-throughput 384-well format. Medium composition affected spheroid histology, global proteome profile, drug metabolism and drug-induced toxicity. No epithelial-mesenchymal transition was observed. Media with fasting glucose and insulin levels gave spheroids with phenotypes closest to normal PHH. The most expensive medium resulted in PHH features most divergent from that of native PHH. Our results provide a protocol for culture of healthy PHH with maintained function - a prerequisite for studies of hepatocyte homeostasis and more reproducible hepatocyte research.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551077 | PMC |
| http://dx.doi.org/10.1016/j.isci.2021.103235 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The role of the NOTCH1 signaling pathway in the maintenance of mesenchymal stem cell stemness and chondrocyte differentiation and its potential in the treatment of osteoarthritis.
J Orthop Surg Res
January 2025
Department of Knee Surgery, The First Hospital of Hebei Medical University, Hebei, China.
Objective: This study aims to explore the potential role of mesenchymal stem cells (MSCs) in the treatment of osteoarthritis (OA), particularly the function of the NOTCH1 signaling pathway in maintaining the stemness of MSCs and in chondrocyte differentiation.
Methods: Utilizing diverse analytical techniques on an osteoarthritis dataset, we unveil distinct gene expression patterns and regulatory relationships, shedding light on potential mechanisms underlying the disease. Techniques used include the culture of MSCs, induction of differentiation into chondrocytes, establishment of stable cell lines, Western Blot, and immunofluorescence.
Designing and incorporating integrated oral health care pathways for pediatric and adult patients: touchpoints in a dental setting.
BMC Oral Health
January 2025
National Center for Professional Training, Ministry of Health and Medical Education, Tehran, Iran.
Background: Maintenance of oral health, prevention, and health promotion stand as primary competencies for dental graduates. Consequently, it is necessary to promote such an approach in dental schools, which are traditionally focused on treatment, to improve the attitude and practice of students in the field of prevention, the final result of which is the reduction of oral and dental diseases in patients. The study aimed to design Integrated Oral Health Care Pathways (IOHCPs) for adults and children referred to Tehran University of Medical Sciences (TUMS), School of Dentistry.
View Article and Find Full Text PDFRisk-based bridge life cycle cost and environmental impact assessment considering climate change effects.
Sci Rep
January 2025
Department of Civil and Environmental Engineering, Seoul National University, Seoul, 08826, Republic of Korea.
To enhance sustainability and resilience against climate change in infrastructure, a quantitative evaluation of both environmental impact and cost is important within a life cycle framework. Climate change effects can lead performance deterioration in bridge components during their operational phase, highlighting the necessity for a risk-based evaluation process aligned with maintenance strategies. This study employs a two-phase life cycle assessments (LCA) framework.
View Article and Find Full Text PDFOptimal allocation of technical reclamation and ecological restoration for a cost-effective solution in Pingshuo Opencast Coal Mine area of China.
J Environ Manage
January 2025
School of Land Science and Technology, China University of Geosciences, 29 Xueyuan Road, Haidian District, 100083, Beijing, People's Republic of China.
Limiting adverse consequences of mining activities requires ecosystem restoration efforts, whose arrangement around mining areas is poorly designed. It is unclear, however, where best to locate ecological projects to enhance ecosystem services cost-effectively. To answer this question, we conducted an optimized ecological restoration project planning by the Resource Investment Optimization System (RIOS) model to identify the restoration priority areas in the Pingshuo Opencast Coal Mine region in Shanxi Province.
View Article and Find Full Text PDFA comprehensive review of postharvest quality and non-thermal preservation techniques for functional mushrooms.
Crit Rev Food Sci Nutr
January 2025
Division of Agricultural Engineering, ICAR-Indian Agricultural Research Institute, New Delhi, India.
Health concerns are increasingly prevalent due to aging populations and lifestyle-related diseases. Concurrently, modern consumers seek natural alternatives and are wary of medication side effects, emphasizing the importance of natural compounds for health maintenance. Functional mushrooms, known for their adaptogenic properties, offer health benefits beyond nutrition and are valued as nutraceuticals and functional foods.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!