In-situ fermentation coupling with partial-denitrification/anammox process for enhanced nitrogen removal in an integrated three-stage anoxic/oxic (A/O) biofilm reactor treating low COD/N real wastewater.

Mainstream partial-denitrification with anammox (PD-anammox) process faced the challenge of complex organics involved in real sewage. Herein, PD-anammox coupled with in-situ fermentation was successfully achieved in a full biofilm system formed by three-stage anoxic/oxic reactor to treat real wastewater with low COD/N of 3.6. The total nitrogen (TN) removal efficiency was enhanced to 78.4% ± 3.6% with average TN and ammonium concentrations in effluent of 10.6 and 0.5 mg N/L, respectively. Batch tests confirmed that partial-denitrification was the major nitrite provider for anammox in the anoxic biofilm, while in-situ fermentation could decompose the complex organics to readily-biodegradable organics for full- or partial-denitrification. Additionally, a significant anammox bacteria (Candidatus Brocadia) population was detected in the second (3.53%) and third (4.46%) anoxic zones, while denitrifiers and fermentative bacteria were mainly enriched in the first anoxic zone. This study presents a feasible approach for PD-anammox process in practical application under mainstream condition.