The aim of the current study was to investigate the effect of well-characterized TiO nanoparticles on DNA methylation of peripheral blood mononuclear cells (PBMCs) . Maximum non-toxic concentration of nanoparticles for PBMCs was determined by MTT assay. The effect of TiO nanoparticles at concentrations of 25-100 μg/ml on DNA methylation of PBMCs was investigated by measuring the %5-mC alterations through an ELISA assay. The physicochemical analysis showed that the TiO nanoparticles were crystalline, pure and in the anatase phase. Peaks related to Ti-O tensile vibrations were observed in the range of 1510 cm. The size of nanoparticles was in the range of 39-74 nm with an average hydrodynamic diameter of 43.82 nm. According to the results of the MTT test, 100 μg/ml was found to be maximum non-toxic concentration. The %5-mC in treated PBMCs revealed that TiO nanoparticles could lead to DNA hypomethylation in PBMCs. The %5-mC difference compared with the negative control was found to be 2.07 ± 1.02% ( = 0.03). The difference of %5-mC between the 25 and 100 μg/ml concentration of nanoparticles was statistically significant ( = 0.02). The results of the current study show that the TiO nanoparticles cause DNA hypomethylation in PBMCs in a dose-response manner. Therefore, it is recommended to evaluate the effects of cytotoxicity and epigenotoxicity of commonly used nanoparticles before their use.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557643 | PMC |
| http://dx.doi.org/10.1093/toxres/tfab085 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Titanium dioxide -mediated regulation of enzymatic and non-enzymatic antioxidants, pigments, and diosgenin content promotes cold stress tolerance in Trigonella foenum-graecum L.
Sci Rep
January 2025
Plant Production Engineering and Genetics Department, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.
Abiotic stresses, notably cold stress, significantly influence various aspects of plant development and reproduction. Various approaches have been proposed to counteract the adverse impacts of cold stress on plant productivity. The unique properties of nanoparticles contribute to an enhanced tolerance of plants to challenging conditions.
View Article and Find Full Text PDFEnhanced bone cement for fixation of prosthetic joint utilizing nanoparticles.
J Mater Sci Mater Med
January 2025
Biomedical Engineering Department, Faculty of Engineering, Helwan University, Cairo, Egypt.
Bone cement is commonly utilized to secure prosthetic joints in the body because of its robust fixation, stability, biocompatibility, and immediate load-bearing capability. However, issues such as loosening, leakage, and insufficient bioactivity can lead to its failure. Therefore, improving its mechanical, physical, and biological properties is crucial for enhancing its efficiency.
View Article and Find Full Text PDFFabrication and characterization of chitosan-based bionanocomposite coating reinforced with TiO nanoparticles and carbon quantum dots for enhanced antimicrobial efficacy.
Int J Biol Macromol
January 2025
Department of Materials Engineering, Materials and Energy Research Center, Dezful Branch, Islamic Azad University, Dezfool, Iran.
Polymer-based nanocomposite coatings that are enhanced with nanoparticles have gained recognition as effective materials for antibacterial purposes, providing improved durability and biocidal effectiveness. This research introduces an innovative chitosan-based polymer nanocomposite, enhanced with titanium oxide nanopowders and carbon quantum dots. The material was synthesized via the sol-gel process and applied to 316L stainless steel through dip-coating.
View Article and Find Full Text PDFSynthesis, characterization and antimicrobial application of carrageenan/TiO composite materials.
Int J Biol Macromol
January 2025
Department of Chemistry, College of Natural and Mathematical Sciences, The University of Dodoma, P.O Box 259, Dodoma, Tanzania. Electronic address:
In this study, a highly crystalline anatase/rutile mixed phase carrageenan/TiO nanocomposite with a larger surface area was synthesized via the sol-gel process and calcined at 450 °C and 650 °C. The synthesized composite materials were characterized by FTIR, XRD, SEM, EDX, TEM, BET and TGA. FTIR confirms the presence of C-Ti-O bond formation in composite.
View Article and Find Full Text PDFPoly(Acrylic Acid)/TiO Nanocomposite Hydrogels for Paper Artwork Cleaning and Protection.
Molecules
December 2024
Department of Chemical Engineering Materials Environment, Sapienza University of Rome, Via del Castro Laurenziano 7, 00161 Rome, Italy.
Paper-based artworks are prone to natural aging processes driven by chemical and biological processes. Numerous treatments have been developed to mitigate deterioration and prevent irreversible damage. In this study, we investigated the use of poly(acrylic acid)/TiO composite hydrogels, combining their cleaning and protective functions in a minimally invasive treatment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!