AI Article Synopsis
- BAF57 is a transcription factor that has been studied in cancers like prostate, breast, and ovarian, but its role in colorectal cancer (CRC) has not been clear, prompting this investigation.
- The study analyzed BAF57 expression in CRC cell lines and clinical specimens, finding that higher levels of BAF57 were linked to decreased overall and recurrence-free survival rates in patients.
- The results suggest that BAF57 is associated with cancer severity and could serve as a potential new target for CRC treatment.
Article Abstract
Background/aim: The role of brahma-related gene 1 (BRG1)-associated factor 57 (BAF57), a transcription factor, has been determined in prostate, breast, and ovarian cancer. However, the relationship between BAF57 and colorectal cancer (CRC) is obscure. Thus, we examined the functional correlation between BAF57 expression and oncological malignancy in CRC in vitro.
Materials And Methods: BAF57 expression in WiDr and HT29 CRC cell lines and clinical specimens from CRC patients was analysed by western blotting and/or RT-PCR. BAF57 expression was down-regulated in WiDr cells through siRNA transfection. An invasion assay was also performed to assess malignancy.
Results: BAF57 was expressed in both human CRC cell lines. Overall survival and recurrence-free survival rates were significantly reduced in high BAF57-expressing specimens. BAF57 expression was an independent predictive factor for long-term survival.
Conclusion: BAF57 correlates with oncological malignancy and may be a novel therapeutic target in CRC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.21873/anticanres.15356 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assembly and interaction of core subunits of BAF complexes and crystal study of the SMARCC1/SMARCE1 binary complex.
Biochem Biophys Res Commun
April 2022
Department of Epidemiology and Biostatistics, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, China. Electronic address:
The multi-subunit ATP-dependent chromatin remodeling factor SWI/SNF complex is a fundamental regulator of gene transcription. The SWI/SNF complex in mammals, also called the BAF complex, consists of 9-12 subunits. Genomic functional studies have found that 20%-25% of human cancers are caused by mutations in genes encoding this complex.
View Article and Find Full Text PDFBAF57 Is a Potential Determinant of Colorectal Cancer Malignancy.
Anticancer Res
November 2021
Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
Article Synopsis
- BAF57 is a transcription factor that has been studied in cancers like prostate, breast, and ovarian, but its role in colorectal cancer (CRC) has not been clear, prompting this investigation.
- The study analyzed BAF57 expression in CRC cell lines and clinical specimens, finding that higher levels of BAF57 were linked to decreased overall and recurrence-free survival rates in patients.
- The results suggest that BAF57 is associated with cancer severity and could serve as a potential new target for CRC treatment.
SWI/SNF deficient central nervous system neoplasms.
Semin Diagn Pathol
May 2021
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, United States. Electronic address:
The SWItch/Sucrose Non-Fermentable (SWI/SNF) complexes are ubiquitous ATP dependent chromatin remodeling complexes that provide epigenetic regulation of gene expressions across the genome. Different combination of SWI/SNF subunits allow tissue specific regulation of critical cellular processes. The identification of SMARCB1 inactivation in pediatric malignant rhabdoid tumors provided the first example that the SWI/SNF complex may act as a tumor suppressor.
View Article and Find Full Text PDFBAF57/SMARCE1 Interacting with Splicing Factor SRSF1 Regulates Mechanical Stress-Induced Alternative Splicing of Cyclin D1.
Genes (Basel)
February 2021
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 401120, China.
Cyclin D1 regulates cyclin-dependent protein kinase activity of the cell cycle, and alternative splicing generates a isoform, acting as a mediator of aberrant cellular proliferation. As alternative splicing processes are sensitive to mechanical stimuli, whether the alternative splicing of is regulated by mechanical stress and what kinds of factors may act as the regulator of mechano-induced alternative splicing remain unknown. The alternative splicing of was examined using reverse transcription polymerase chain reaction (RT-PCR) in osteoblast cell lines and keratinocyte cells loaded by a cyclic stretch.
View Article and Find Full Text PDFThe ATPase BRG1/SMARCA4 is a protein interaction platform that recruits BAF subunits and the transcriptional repressor REST/NRSF in neural progenitor cells.
Mol Cell Biochem
November 2019
Department of Biomedicine, Aarhus University, Wilhelm Meyers Allé 3, Building 1233/1234, 8000, Aarhus C, Denmark.
The BAF complex (SWI/SNF) is an ATP-dependent chromatin remodeler that adapts the structural organization of the chromatin. Despite a growing understanding of the composition of BAF in different cell types, the interaction network within the BAF complex is poorly understood. Here, we characterized an isoform of the BRG1/SMARCA4 ATPase expressed in human neural progenitor cells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!