AI Article Synopsis
- Epstein-Barr virus (EBV) can lead to cancers in some infected individuals, but its effects on humoral immunity among cancer-free adults are still not fully understood.
- A study analyzed serum samples from 316 adults (175 Taiwanese and 141 Northern European) using a protein microarray to observe IgG antibody responses to various EBV peptide sequences.
- Eight EBV peptide sequences were identified as significant for B-cell immunity, involving three specific proteins linked to IgG reactivity and five with variant amino acids that also influence immune response.
Article Abstract
Background: Epstein-Barr virus (EBV) infection contributes to cancers in a fraction of seropositive individuals, but much remains to be learned about variation in EBV-directed humoral immunity in cancer-free adults.
Methods: A protein microarray was used to probe serum from 175 Taiwanese and 141 Northern European adults for immunoglobulin G (IgG) antibody responses to 115 different peptide sequences, representing protein segments or protein variants, from 45 EBV proteins. It was posited that this antibody-based approach could identify EBV peptide sequences representing immunodominant regions relevant for B-cell immunity.
Results: Analyses of 45 EBV proteins with multiple protein segments or variants printed on the array identified eight EBV peptide sequences that appear to play a role in immunogenicity. This included: (1) three proteins with segments/regions associated with IgG reactivity (BALF5, LMP1, LMP2A); and (2) five proteins with sequence variants/amino acid changes associated with IgG reactivity (BDLF4, EBNA3A, EBNA3B, EBNA-LP, LF1).
Conclusion: This examination of IgG antibody responses against 115 EBV peptide sequences in 316 cancer-free adults represents an important step toward identifying specific EBV protein sequences that play a role in generating B-cell immunity in humans.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8724419 | PMC |
| http://dx.doi.org/10.1016/j.ijid.2021.10.054 | DOI Listing |
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