[Role of miR-145-5p Targeting ARK5 in Regulating the Proliferation and Apoptosis of Human Epithelial Ovarian Cancer Cells].

Objective To explore the effect of miR-145-5p on the proliferation and apoptosis of human ovarian cancer cells and the possible molecular mechanisms involved.Methods Real-time quantitative PCR was performed to detect the expression of miR-145-5p in ovarian epithelial cells and ovarian cancer cells.CCK-8 and flow cytometry were used to detect the effects of miR-145-5p overexpression on the proliferation and apoptosis of ovarian cancer cells.TargetScan was employed to predict the target genes of miR-145-5p.Western blotting,dual luciferase reporter assay and rescue experiment were employed to predict and verify the underlying molecular mechanism of miR-145-5p function.Results The expression of miR-145-5p in ovarian cancer cells was significantly lower than that in normal ovarian epithelial cells(=4.345,=0.049).Compared with the control group,the overexpression of miR-145-5p reduced the proliferation rate(=-15.790,<0.001)and increased the apoptosis rate(=5.433,=0.032)of ovarian cancer cells.ARK5 was predicted as the direct target gene of miR-145-5p(=4.583,=0.010).The cells with ARK5 overexpression showed increased proliferation rate(=27.290,<0.001)and decreased apoptosis rate(=-8.241,=0.001).The overexpression of miR-145-5p can down-regulate the mRNA(=-12.824,<0.001)and protein(=-4.792,=0.001)levels of ARK5.The rescuing expression of ARK5 significantly offset the inhibitory effects of miR-145-5p on cell proliferation(=15.580,=0.004)and apoptosis(=-12.470,=0.006).Conclusion miR-145-5p may inhibit the proliferation and promote the apoptosis of ovarian cancer cells by targeting ARK5.