AI Article Synopsis
- Malaria infection occurs when a mosquito injects sporozoites into a host's skin, and passive immunization with antibodies against mosquito saliva can protect mice from infection.
- The study focused on creating monoclonal antibodies against the mosquito protein AgTRIO, identifying a significant protective region in the protein.
- Monoclonal antibody 13F-1 notably reduced infection in mice and is crucial for developing better malaria vaccines that incorporate elements from both the malaria pathogen and its mosquito vector.
Article Abstract
Malaria begins when an infected mosquito injects saliva containing sporozoites into the skin of a vertebrate host. Passive immunization of mice with antiserum against the Anopheles gambiae mosquito saliva protein TRIO (AgTRIO) offers significant protection against infection of mice. Furthermore, passive transfer of both AgTRIO antiserum and an anti-circumsporozoite protein monoclonal antibody provides synergistic protection. In this study, we generated monoclonal antibodies against AgTRIO to delineate the regions of AgTRIO associated with protective immunity. Monoclonal antibody 13F-1 markedly reduced infection in mice and recognized a region (VDDLMAKFN) in the carboxyl terminus of AgTRIO. 13F-1 is an IgG2a isotype monoclonal antibody, and the Fc region is required for protection. These data will aid in the generation of future malaria vaccines that may include both pathogen and vector antigens.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788779 | PMC |
| http://dx.doi.org/10.1128/IAI.00359-21 | DOI Listing |
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