. in Lower Respiratory Tract Secretions - A Ten Years Retrospective Study.

Introduction: Lower respiratory tract secretions (LRTS) like sputum and tracheal aspirates are frequently sent to the microbiology laboratory from patients with various respiratory pathologies. Improper collection techniques can lead to false-positive results, resulting in improper therapy.

Aim Of The Study: To determine the percentage of contaminated samples sent to the microbiology laboratory, to establish the prevalence of spp. in non-contaminated samples and therefore, the presence of spp. originating in lower respiratory tract infections. Material and.

Methods: A 10-year data survey was conducted to assess the differences in prevalence from contaminated versus non-contaminated samples, assessed and categorised by Bartlett grading system, and to emphasise the importance of quality control for potentially contaminated samples. The data were analysed according to gender, age, referring departments, and the species of . For the statistical analysis, Kruskal-Wallis and Fisher tests were used, and the alpha value was set for 0.5.

Results: The prevalence of spp. in all analysed samples was 31.60%. After excluding the contaminated samples, the actual prevalence was 27.66%. Of all sputum samples, 31.6% were contaminated. Patients aged more than 40 years old were more prone to provide contaminated sputum samples. is more prevalent in non-contaminated sputum samples. In both sputum and tracheal aspirates, the chances of identifying a single species are higher than the chances of identifying multiple species.

Conclusions: The study emphasises the importance of assessing the quality of sputum samples because of the high number of improperly collected samples sent to the microbiology laboratory.