AI Article Synopsis
- Proper gene expression is crucial for the development of mammalian skeletal muscle, influenced by both mRNA and long non-coding RNAs (lncRNAs).
- The study investigates N-methyladenosine (mA) modifications in lncRNAs throughout muscle development using RNA sequencing techniques.
- Findings show that lncRNA expression varies over time and that mA methylation levels positively correlate with these lncRNA expression levels, indicating a potential link between mA and the regulation of nearby mRNAs.
Article Abstract
Proper development of mammalian skeletal muscle relies on precise gene expression regulation. Our previous studies revealed that muscle development is regulated by both mRNA and long non-coding RNAs (lncRNAs). Accumulating evidence has demonstrated that N-methyladenosine (mA) plays important roles in various biological processes, making it essential to profile mA modification on a transcriptome-wide scale in developing muscle. Patterns of mA methylation in lncRNAs in developing muscle have not been uncovered. Here, we reveal differentially expressed lncRNAs and report temporal mA methylation patterns in lncRNAs expressed in mouse myoblasts and myotubes by RNA-seq and methylated RNA immunoprecipitation (MeRIP) sequencing. Many lncRNAs exhibit temporal differential expression, and mA-lncRNAs harbor the consensus mA motif "DRACH" along lncRNA transcripts. Interestingly, we found that mA methylation levels of lncRNAs are positively correlated with the transcript abundance of lncRNAs. Overexpression or knockdown of mA methyltransferase METTL3 alters the expression levels of these lncRNAs. Furthermore, we highlight that the function of mA genic lncRNAs might correlate to their nearby mRNAs. Our work reveals a fundamental expression reference of mA-mediated epitranscriptomic modifications in lncRNAs that are temporally expressed in developing muscle.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548731 | PMC |
| http://dx.doi.org/10.3389/fcell.2021.762669 | DOI Listing |
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