Pumpkin () is an important cucurbit vegetable crop that has strong resistance to abiotic stress. While heat shock protein 20 (HSP20) has been implicated in vegetable response to heat stress, little is known regarding activity of HSP20 family proteins in . Here, we performed a comprehensive genome-wide analysis to identify and characterize the functional dynamics of the () gene family. A total of 33 genes distributed across 13 chromosomes were identified from the pumpkin genome. Our phylogenetic analysis determined that the CmoHSP20 proteins fell into nine distinct subfamilies, a division supported by the conserved motif composition and gene structure analyses. Segmental duplication events were shown to play a key role in expansion of the gene family. Synteny analysis revealed that 19 and 18 genes were collinear with those in the cucumber and melon genomes, respectively. Furthermore, the expression levels of pumpkin genes were differentially induced by heat stress. The transcript level of , and were down-regulated by heat stress, while , , , , and were up-regulated by heat stress, which could be used as heat tolerance candidate genes. Overall, these findings contribute to our understanding of vegetable HSP20 family genes and provide valuable information that can be used to breed heat stress resistance in cucurbit vegetable crops.
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| http://dx.doi.org/10.3389/fgene.2021.753953 | DOI Listing |
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