Wound Healing Effectivity of the Ethanolic Extracts of L. Leaf (White and Purple Flower Type) and and Gel Preparation in Animal Model.

Unlabelled: The study's objective was to determine the wound healing activity of the combination of ethanolic extracts of L. leaf (white and purple), , and astaxanthin gel preparation.

Materials And Methods: For in-gel preparation, three different formulas of gelling agents, namely carbopol 934 (1%), hydroxypropyl methylcellulose (HPMC) (9%), and natirum-carboxymethylcellulose (Na-CMC) (4%), were employed. Then, the organoleptic, pH, spreadability, and viscosity of the formulas were evaluated. To determine wound healing activity, six treatments, including negative control (placebo), positive control (bioplacenton), BP5 ( L. leaf ethanolic extract of white flower type 5%, L. Urb leaf ethanolic extract 2.5%, astaxanthin 0.05%), BU5 ( L. leaf ethanolic extract of purple flower type 5%, L. Urb leaf ethanolic extract 2.5%, astaxanthin 0.05%), BU10 ( L. leaf ethanolic extract of purple flower type 10%, L. Urb leaf ethanolic extract 5%, and astaxanthin 0.1%), and BP10 ( L. leaf ethanolic extract of white flower type 10%, L. Urb leaf ethanolic extract 5%, and astaxanthin 0.1%) were evaluated. All treatments were applied to an incision wound (1.5 cm). Measurement of the wound length was conducted daily for 14 days.

Results: The results showed that the carbopol 934 (1%) gelling agent formula was better than HPMC and Na-CMC. Meanwhile, the percentages of wound healing activity for negative, positive, BP5, BU5, BU10, and BP10 groups were 72.51%, 69.36%, 70.14%, 81.70%, 86.54%, and 80.21%, respectively. The BU5 and BU10 showed significant activity (p<0.05) compared with positive and negative controls.

Conclusion: BU10 provided the best wound healing activity and can be developed as a commercial product.