AI Article Synopsis
- Flow cytometry is an advanced technology for identifying and characterizing cells, widely used in research and clinical settings, that can analyze multiple targets simultaneously from small samples.
- Recent improvements in technology have allowed for more complex flow cytometry panels, which can detect a wider variety of cell markers and sub-populations but also complicate panel design and data analysis.
- The paper discusses established practices for creating high-dimensional panels using the Aurora spectral flow cytometer to enhance the analysis of clinical samples.
Article Abstract
Flow cytometry is a powerful technology used in research, drug development and clinical sample analysis for cell identification and characterization, allowing for the simultaneous interrogation of multiple targets on various cell subsets from limited samples. Recent advancements in instrumentation and fluorochrome availability have resulted in significant increases in the complexity and dimensionality of flow cytometry panels. Though this increase in panel size allows for detection of a broader range of markers and sub-populations, even in restricted biological samples, it also comes with many challenges in panel design, optimization, and downstream data analysis and interpretation. In the current paper we describe the practices we established for development of high-dimensional panels on the Aurora spectral flow cytometer to aid clinical sample analysis.
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| http://dx.doi.org/10.4155/bio-2021-0201 | DOI Listing |
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