Phytochemical Profile and Antioxidant and Antiproliferative Activity of on Pterygium Fibroblasts.

Background: has antioxidant effects that are beneficial for different diseases. We aimed to analyze the antiproliferative activity of in human pterygium fibroblasts (HPFs).

Methods: HPFs were treated for 24 h with 0-1000 g/mL of lyophilized to analyze its effect on cell viability using the CellTiter assay. RNA from HPF treated with 250 g/mL of lyophilized was isolated, and the expression of VEGF and CTGF genes was evaluated by qPCR. A dermal fibroblast cell line (HDFa) was used as a healthy control. The total phenolic content, antioxidant activity, and chemical profile of lyophilized were determined.

Results: Viability of HPF decreased after 24 h treatment of in a dose-dependent manner. The expression of VEGF and CTGF significantly decreased ( < 0.01) in HPF treated with 250 g/mL of when compared with untreated HPF. The total phenolic concentration in the lyophilized was 33.67 mg gallic acid equivalents (GAE)/g. Antioxidant activity was 384.49 mM Trolox equivalents/mL. The main phenolic compounds identified by HPLC analysis were the kaempferol-3-O-glycoside, kaempferol-3-O-rhamnoside, kaempferol-3-O-neohesperidoside-7-O--rhamnopyranoside, and kaempferol-3-O-glycoside-7-O-rhamnoside.

Conclusions: decreases the proliferation of HPF and the expression of VEGF and CTGF. The phenolic compound concentration, antioxidant activity, and phytochemical profile may play a role in these effects.