An effective approach to extracting four flavonoids (lysioside C, nevadensin-7-sambubioside, ikarisoside B, and nevadensin) from using ultrasonic-assisted aqueous two-phase extraction (UAATPE) technology was studied. Ethanol/KHPO system was selected and the influence of several important parameters, including composition of the aqueous two-phase system (ATPS), extraction temperature and time, particle size, and solvent to material ratio, were investigated by single factor experimentss. Then three key parameters (ethanol concentration, solvent to material ratio and extraction temperature) were further optimized by response surface methodology (RSM). The optimal process was that 1 g 120 mesh herb powders were extracted with 45 g ATPS (made of 30% ethanol/18% KHPO) in 43 °C for 30 min, and yields of four flavonoids could reach 2.56, 2.06, 3.62, and 6.28 mg/g, respectively. Compared with ultrasonic-assisted extraction (UAE) in three conventional solvents (water, ethanol and 60% ethanol), the UAATPE displayed comparatively higher extraction capability. Therefore, UAATPE in ethanol/KHPO system could be an alternative technology for integrated extraction of the flavonoids from .
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