Gut Microbiota-Mediated Immunomodulatory Effects of HDB1258 Cultured in the Lava Seawater in the Colitis Mouse Model.

The immunomodulatory effects of HDB1258 were evaluated in mice with colitis induced by (KO). HDB1258 was cultured in the lava seawater (LS) to improve its probiotic properties. It increased adhesive ability to mucin with mRNA expression levels of chaperone proteins (such as GroEL/ES, DnaKJ, and HtrA). In the experiments, administration of KO caused an inflammation on the colon with gut dysbiosis. LH group (oral gavage of HDB1258 1.0 × 10 colony forming units/day) showed that inflammatory biomarkers, including IL-1, TNF-, IL-6, and PGE, were significantly decreased to less than half of the KO group, and Th1 cells were decreased in the spleen, but Treg cells were not affected. In contrast, the expression levels of secretory IgA and IL-10 were significantly increased, and the composition of gut microbiota in the LH group tended to recover similar to normal mice without any effect on the -diversity. In conclusion, HDB1258 cultured in the LS could regulate competitively pathogenic bacteria in imbalanced flora with its improved mucin adhesive ability and was an effective immunomodulatory adjuvant for treating colitis by its regulatory function on intestinal inflammation.