AI Article Synopsis
- A protocol is introduced to manipulate the phase separation capacity of proteins, specifically focusing on mutations in intrinsically disordered regions (IDRs).
- By mutating specific residues, the study can disrupt phase separation, which is crucial for cell fate transitions.
- The researchers also explore ways to restore phase separation by fusing IDRs known to drive it, highlighting the challenge of predicting which mutations will be effective.
Article Abstract
Phase separation of proteins regulates transcription. Here, we present a protocol to manipulate phase separation capacity of a protein. We use this protocol to disrupt phase separation by mutating residues at intrinsically disordered regions (IDRs). Further, we rescue the disabled phase separation by fusing an IDR known to drive phase separation. Phase separation promotes cell fate transitions, whereas disruption of phase attenuates the transitions. The major challenge is how to effectively predict mutation residues. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8524243 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.100887 | DOI Listing |
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