lncRNA suppresses metastasis and chemo-resistance by modulating the phosphorylation of c-Jun protein in GBC.

Long non-coding RNAs (lncRNAs) have been identified as critical contributors in tumor progression for many types of cancer. However, their functions in gallbladder cancer (GBC) have not been systematically clarified. In this study, the clinical significance, biological function, and underlying mechanism of lncRNA in GBC were investigated. The quantitative real-time PCR result indicated that lncRNA was found to be recurrently downregulated in GBC tumor samples. Kaplan-Meier analysis revealed that decreased lncRNA expression level was associated with poor survival of GBC patients (p = 0.025). Then, both and experiments elucidated that the overexpression of lncRNA suppressed the migration and invasion abilities of GBC cells and promoted the sensitivity to gemcitabine of GBC cells. Furthermore, we found that lncRNA physically interacted with c-Jun protein and decreased the phosphorylation on serine-73 (c-Jun-Ser73), which might cause the enhancement of the migration, invasion, and sensitivity to gemcitabine of GBC tumor cells. In conclusion, our study identified lncRNA as a promising prognostic indicator for patients with GBC, providing insights into the molecular pathogenesis of GBC. lncRNA is a potential therapeutic combination for gemcitabine in GBC treatment.