Pseudogene fms-related tyrosine kinase 1 pseudogene 1 (FLT1P1) cooperates with RNA binding protein dyskeratosis congenita 1 (DKC1) to restrain trophoblast cell proliferation and angiogenesis by targeting fms-related tyrosine kinase 1 (FLT1) in preeclampsia.

In preeclampsia (PE), preexistent maternal endothelial dysfunction leads to impaired placentation and vascular maladaptation. Long noncoding RNAs (lncRNAs) have been shown to participate in the placentation process. LncRNA fms-related tyrosine kinase 1 pseudogene 1 () was previously reported to be upregulated in PE. In this study, we verified the effect of and its cognate gene on trophoblast cell proliferation and angiogenesis by using Cell Counting Kit-8 (CCK-8) assay, tube formation assay, and western blot analysis. Their binding to RNA binding protein dyskeratosis congenita 1 () was validated by conducting RNA immunoprecipitation (RIP) and RNA pulldown assays. In this study, knockdown of or was found to promote cell proliferation and angiogenesis in trophoblasts. In addition, recruited to stabilize . Importantly, silencing or decreased the stability of . Rescue assays revealed that overexpression reversed the effect of silenced . Overall, cooperates with to restrain trophoblast cell proliferation and angiogenesis by targeting .