AI Article Synopsis
- The study focuses on a medicinal plant known for accumulating camptothecin (CPT), a key compound used in chemotherapy for cancer treatment.
- Researchers analyzed the TCP transcription factor family and identified 16 genes related to CPT biosynthesis, classifying them into two subgroups based on genetic relationships.
- Experiments showed that most TCP genes are highly expressed in leaves and stems, suggesting they play a significant role in regulating CPT production, which could lead to new strategies for enhancing CPT yield.
Article Abstract
(; ) is a medicinal herbaceous plant, which can accumulate camptothecin (CPT). CPT and its derivatives are widely used as chemotherapeutic drugs for treating malignant tumors. Its biosynthesis pathway has been attracted significant attention. Teosinte branched 1/cycloidea/proliferating cell factors 1/2 (TCP) transcription factors (TFs) regulate a variety of physiological processes, while TCP TFs are involved in the regulation of CPT biosynthesis remain unclear. In this study, a systematic analysis of the TCP TFs family in was performed. A total of 16 TCP () genes were identified and categorized into two subgroups based on their phylogenetic relationships with those in . Tissue-specific expression patterns revealed that nine genes showed the highest expression levels in leaves, while the other seven showed a higher expression level in the stems. Co-expression, phylogeny analysis, and dual-luciferase (Dual-LUC) assay revealed that potentially plays important role in CPT and its precursor biosynthesis. In addition, the subcellular localization experiment of candidate genes showed that they are all localized in the nucleus. Our study lays a foundation for further functional characterization of the candidate genes involved in CPT biosynthesis regulation and provides new strategies for increasing CPT production.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529195 | PMC |
| http://dx.doi.org/10.3389/fpls.2021.746648 | DOI Listing |
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