Purpose: The healthy human urine sediment proteome and metaproteome are investigated, to shed light on the variations of urine sediment proteins and metaproteins associated with sex and age.
Experimental Design: Urine sediment samples are collected from 19 healthy subjects. Protein identification and quantification are performed by liquid chromatography coupled high-resolution mass spectrometry.
Results: A total of 2736 human proteins were identified, which were primarily associated with inflammatory response and energy metabolism. For the metaproteome, 65 genera were identified that were primarily involved in translation and carbohydrate metabolic processes. The median biological coefficient variation of the proteome/metaproteome of human urine sediment was 0.5/0.72, similar to the proteome of human urine supernatant. In addition, sex and age were observed to affect the proteome and metaproteome of healthy human urine sediment.
Conclusion And Clinical Relevance: The healthy human urine sediment were characterized, indicating that urine sediment might represent an alternative resource for disease research in addition to urine supernatant, but the influence of sex and age must be considered in the study design process.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/prca.202100007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cell-Free Carbonic Anhydrase IX mRNA in Urine as Biomarker for Urogenital Cancers: The Relationship Between Urinary Extracellular RNA and Tumor-Cell-Associated RNA.
Curr Issues Mol Biol
December 2024
Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Gaetano Pieraccini 6, 51039 Florence, Italy.
Circulating tumor cells and cell-free nucleic acids are novel diagnostic, prognostic and predictive tools for non-invasive and cost-effective cancer detection in liquid biopsy. Carbonic anhydrase IX (CAIX) has been proposed as a biomarker in urogenital tumors and urine sediment. Our aim was to evaluate CAIX full-length percentage (CAIX FL%) in urine-cell-free RNA (cfRNA) and its relationship with tumor-cell-associated RNA (TC-RNA).
View Article and Find Full Text PDFCrab urine as biomonitoring tools for pollution: A state-of-the-art review of methods, organic contaminant levels, and comparisons between spilled and unspilled areas.
Mar Pollut Bull
December 2024
Laboratory for Assessment of Organic Contaminants (LACOr), Institute of Marine Sciences-Federal University of Ceará (LABOMAR-UFC), Av. Abolição, 3207-Meireles, CEP: 60165-081 Fortaleza, CE, Brazil; Tropical Marine Sciences Program (PPGCMT/LABOMAR/UFC), Brazil; Programa de Pós-graduação em Ciências Naturais/Ceará State University (PPGCN/UECE), Brazil. Electronic address:
Article Synopsis
- This study systematically reviews methods for extracting and analyzing organic contaminants in crab urine, which are essential due to crabs' role as bioindicators of aquatic contamination.
- Although harmful substances like PCBs and pesticides are known to affect ecosystems, most studies have focused mainly on PAHs, leaving a significant research gap.
- The perception that collecting crab urine is difficult, along with insufficient methodologies in existing literature, highlights the need for more extensive research to improve ecological understanding and environmental monitoring efforts.
Investigating the incremental value of urine sediment reporting in emergency medicine with a Sysmex UN urinalysis system.
Adv Lab Med
December 2024
Section of Clinical Biochemistry and School of Medicine, University of Verona, Verona, Italy.
Objectives: Urinalysis is widely used and is also frequently requested in emergency situations for screening hypovolemia, urinary tract infections, diabetes, ketoacidosis and hematuria. Our aim was to evaluate the impact of reporting urinary sediment in emergency department specimens with the Sysmex UN system.
Methods: We evaluated urinalyses requested by the emergency department over a three-month period and examined red blood cell count interference, compared leukocyte esterase dipsticks to cytofluorimetric leukocyte count and nitrites to cytofluorimetric bacterial count.
RENAL FUNCTION PARAMETERS OF HEALTHY CAPTIVE AND SEMICAPTIVE AMAZONIAN MANATEES ().
J Zoo Wildl Med
December 2024
Laboratory of Aquatic Mammals, National Institute of Amazonian Research-INPA, Manaus, AM 69060-001, Brazil.
Evaluating renal function is essential for managing captive wild animals, particularly threatened species like the Amazonian manatee () in rehabilitation and prerelease programs. A series of urine diagnostic tests, such as gross appearance, semiquantitative chemical analyses, microscopic review of sediments, and quantitative analyses of urea and creatinine, were performed in 57 free-catch urine samples. On the same occasion, 52 serum samples from the same individuals were analyzed for creatine kinase activity, creatinine, blood urea nitrogen, albumin, sodium, potassium, and chloride concentrations; serum symmetric dimethylarginine (SDMA) was measured for the first time in the species.
View Article and Find Full Text PDFUrinary microbiome profiling as a non-invasive tool for identifying biomarkers in systemic lupus erythematosus and lupus nephritis.
Front Cell Infect Microbiol
December 2024
Department of Clinical Laboratory, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, Jiangsu, China.
Introduction: Bacteriome alterations have been implicated in the pathogenesis of systemic lupus erythematosus (SLE). However, the relationship between SLE and the urinary microbiome remains underexplored. This study aimed to characterize the urinary microbiome of SLE patients using 16S rRNA sequencing and to investigate its correlations with clinical parameters through integrative analyses.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!