AI Article Synopsis
- The study identifies five mRNA variants of the high-affinity K transporter in japonica rice, focusing on their expression and roles under salt stress.
- Analysis showed the main variant had significantly higher transcript levels in roots and shoots compared to others, particularly after 24 hours of salt exposure.
- Two-electrode voltage clamp experiments indicated that the variants expressed in oocytes resulted in smaller sodium currents, contributing to insights on ion homeostasis and salt tolerance in rice.
Article Abstract
In rice, the high-affinity K transporter, , functions as a Na-selective transporter. mRNA variants of have been reported previously, but their functions remain unknown. In this study, five variants () were identified from japonica rice (Nipponbare) in addition to . Absolute quantification qPCR analyses revealed that the transcript level of was significantly higher than other variants in both the roots and shoots. Expression levels of , and some variants, increased after 24 h of salt stress. Two electrode voltage clamp experiments in a heterologous expression system using oocytes revealed that oocytes expressing OsHKT1;3_FL and all of its variants exhibited smaller Na currents. The presented data, together with previous data, provide insights to understanding how family members are involved in the mechanisms of ion homeostasis and salt tolerance in rice.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537747 | PMC |
| http://dx.doi.org/10.3390/plants10102006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multicenter In-House Evaluation of an Amplicon-Based Next-Generation Sequencing Panel for Comprehensive Molecular Profiling.
Mol Diagn Ther
January 2025
Istituto Europeo di Oncologia, IRCCS, Via Adamello 16, 20139, Milan, Italy.
Background: Predicting response to targeted cancer therapies increasingly relies on both simple and complex genetic biomarkers. Comprehensive genomic profiling using high-throughput assays must be evaluated for reproducibility and accuracy compared with existing methods.
Methods: This study is a multicenter evaluation of the Oncomine™ Comprehensive Assay Plus (OCA Plus) Pan-Cancer Research Panel for comprehensive genomic profiling of solid tumors.
Exploring the Potential Effect of GLP1R Agonism on Common Ageing-Related Diseases via Glucose Reduction: A Mendelian Randomization Study.
J Gerontol A Biol Sci Med Sci
January 2025
Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, China.
Background: Glucagon-like peptide-1 receptor agonists (GLP1RAs) are widely used in manageing type 2 diabetes mellitus and weight control. Their potential in treating ageing-related diseases has been gaining attention in recent years. However, the long-term effects of GLP1RAs on these diseases have yet to be fully revealed.
View Article and Find Full Text PDFProteomic Profiling of Potential E6AP Substrates via Ubiquitin-based Photo-Crosslinking Assisted Affinity Enrichment.
Chembiochem
January 2025
University of Konstanz, Department of Chemistry, Universitaetsstrasse 10, 78457, Konstanz, GERMANY.
The ubiquitin (Ub) ligase E6AP, which is encoded by the UBE3A gene, has been associated with several human diseases including cervical cancer and Angelman syndrome, a neurodevelopmental disorder. Yet, our knowledge about disease-relevant substrates of E6AP is still limited. The formation of a thioester complex between Ub and the catalytic Cys residue of E6AP represents an essential intermediate step in E6AP-mediated ubiquitination.
View Article and Find Full Text PDFGDBr: genomic signature interpretation tool for DNA double-strand break repair mechanisms.
Nucleic Acids Res
January 2025
Department of Convergent Bioscience and Informatics, College of Bioscience and Biotechnology, Chungnam National University, 99, Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
Large genetic variants can be generated via homologous recombination (HR), such as polymerase theta-mediated end joining (TMEJ) or single-strand annealing (SSA). Given that these HR-based mechanisms leave specific genomic signatures, we developed GDBr, a genomic signature interpretation tool for DNA double-strand break repair mechanisms using high-quality genome assemblies. We applied GDBr to a draft human pangenome reference.
View Article and Find Full Text PDFInpatient Hospitalizations for COVID-19 Among Patients With Prader-Willi Syndrome: A National Inpatient Sample Analysis.
Am J Med Genet A
January 2025
Massachusetts General Hospital, Boston, Massachusetts, USA.
Prader-Willi syndrome (PWS) is a genetic disorder associated with baseline respiratory impairment caused by multiple contributing etiologies. While this may be expected to increase the risk of severe COVID-19 infections in PWS patients, survey studies have suggested paradoxically low disease severity. To better characterize the course of COVID-19 infection in patients with PWS, this study analyses the outcomes of hospitalizations for COVID-19 among patients with and without PWS.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!