AI Article Synopsis
- - The study investigates how intracellular cAMP receptor exchange proteins influence the ability of rickettsial bacteria to adhere to and invade vascular endothelial cells, aiming to clarify the mechanisms involved in this process.
- - Researchers utilized a unique in vivo system combined with fluidic force microscopy to analyze bacterial adhesion, finding that the absence of a specific gene significantly reduces rickettsial binding to endothelial cells.
- - Results indicated that rickettsial protein OmpB has variable binding strength depending on the host, and phosphorylation at a specific site (Y23) plays a crucial role in modulating this adhesion, suggesting that the cAMP signaling pathway is key in rickettsial infection.
Article Abstract
Introduction: Intracellular cAMP receptor exchange proteins directly activated by cAMP 1 () regulate obligate intracellular parasitic bacterium rickettsial adherence to and invasion into vascular endothelial cells (ECs). However, underlying precise mechanism(s) remain unclear. The aim of the study is to dissect the functional role of the signaling pathway during initial adhesion of rickettsiae to EC surfaces.
Methods: In the present study, an established system that is anatomically based and quantifies bacterial adhesion to ECs in vivo was combined with novel fluidic force microscopy (FluidFM) to dissect the functional role of the signaling pathway in rickettsiae-EC adhesion.
Results: The deletion of the gene impedes rickettsial binding to endothelium in vivo. Rickettsial OmpB shows a host -dependent binding strength on the surface of a living brain microvascular EC (BMEC). Furthermore, ectopic expression of phosphodefective and phosphomimic mutants replacing tyrosine (Y) 23 of in -knock out BMECs results in different binding force to reOmpB in response to the activation of .
Conclusions: modulates rickettsial adhesion, in association with Y23 phosphorylation of the binding receptor . Underlying mechanism(s) should be further explored to delineate the accurate role of cAMP- system during rickettsial infection.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537355 | PMC |
| http://dx.doi.org/10.3390/pathogens10101307 | DOI Listing |
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