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Translocated in LipoSarcoma/Fused in Sarcoma (TLS/FUS) is a nuclear RNA binding protein whose mutations cause amyotrophic lateral sclerosis. TLS/FUS undergoes LLPS and forms membraneless particles with other proteins and nucleic acids. Interaction with RNA alters conformation of TLS/FUS, which affects binding with proteins, but the effect of mA RNA modification on the TLS/FUS-RNA interaction remains elusive. Here, we investigated the binding specificity of TLS/FUS to mA RNA fragments by RNA pull down assay, and elucidated that both wild type and ALS-related TLS/FUS mutants strongly bound to mA modified RNAs. TLS/FUS formed cytoplasmic foci by treating hyperosmotic stress, but the cells transfected with mA-modified RNAs had a smaller number of foci. Moreover, mA-modified RNA transfection resulted in the cells obtaining higher resistance to the stress. In summary, we propose TLS/FUS as a novel candidate of mA recognition protein, and mA-modified RNA fragments diffuse cytoplasmic TLS/FUS foci and thereby enhance cell viability.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539258 | PMC |
| http://dx.doi.org/10.3390/ijms222011014 | DOI Listing |
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