Enrichment Free qPCR for Rapid Identification and Quantification of , , , and in Chicken Meat Samples by a New Couple of Primers.

is the main cause of bacterial foodborne disease and poultry meat is the principal source of human infections. Rapid methods for detection are urgently needed to decrease high bacterial prevalence in poultry products. In this study, we developed new primers, CampyPFw and CampyPRv, that target the 16S-23S rRNA genes of , , and The primers were tested on positive and negative reference strains in pure cultures and in inoculated poultry meat samples before their application in real-time PCR (qPCR) protocol for analyzing chicken meat samples. In parallel, the samples were tested by using the ISO 10272-1:2006 method. The qPCR protocol based on CampyPFw and CampyPRv showed good sensitivity, with the limit of detection of 4.6 × 10 cells/mL in chicken samples without enrichment steps.