Controlling spread of resistance genes from wastewater to aquatic systems requires more knowledge on how resistance genes are acquired and transmitted. Whole genomic sequences from sewage-associated staphylococcus isolates (20 , 2 , and 2 ) were analyzed for the presence of antibiotic resistance genes (ARGs) and metal resistance genes (MRGs). Plasmid sequences were identified in each isolate to investigate co-carriage of ARGs and MRGs within. BLASTN analysis showed that 67% of the isolates carried more than one ARG. The carriage of multiple plasmids was observed more in CC5 than CC8 strains. Plasmid exchange was observed in all staphylococcus species except the two isolates that carried multiple MRGs, no ARGs, and no plasmids. 85% of isolates carried the gene, 76% co-carried with and , with 62% of these isolates carrying , , and on the same plasmid. The co-carriage of ARGs and MRGs in isolates, and carriage of MRGs in , without plasmids suggests non-conjugative transmission routes for gene acquisition. More studies are required that focus on the transduction and transformation routes of transmission to prevent interspecies exchange of ARGs and MRGs in sewage-associated systems.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535820 | PMC |
| http://dx.doi.org/10.3390/genes12101473 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Protein phosphatase PP2C2 dephosphorylates transcription factor ZAT5 and modulates tomato fruit ripening.
Plant Physiol
January 2025
College of Horticulture, China Agricultural University, Beijing, 100193, China.
Although C2H2 zinc finger transcription factors are important in plant growth, development, and stress resistance, their specific roles in fruit ripening have been less explored. Here, we demonstrate that the C2H2 zinc finger transcription factor 5 (SlZAT5) regulates fruit ripening in tomato (Solanum lycopersicum L.).
View Article and Find Full Text PDFNovel genomic features in entomopathogenic fungus Beauveria bassiana ILB308: accessory genomic regions and putative virulence genes involved in the infection process of soybean pest Piezodorus guildinii.
Pest Manag Sci
January 2025
Laboratorio de Bioproducción, Bioinsumos, INIA Las Brujas, Canelones, Uruguay.
Background: Biological control methods involving entomopathogenic fungi like Beauveria bassiana have been shown to be a valuable approach in integrated pest management as an environmentally friendly alternative to control pests and pathogens. Identifying genetic determinants of pathogenicity in B. bassiana is instrumental for enhancing its virulence against insects like the resistant soybean pest Piezodorus guildinii.
View Article and Find Full Text PDFResistance reduction and cross-resistance of spinosad-resistant Rhyzopertha dominica (Coleoptera: Bostrichidae) and the association between spinosad resistance and maltase activity.
Pest Manag Sci
January 2025
Department of Entomology, National Chung Hsing University, Taichung City, Taiwan.
Background: The lesser grain borer, Rhyzopertha dominica, is a serious stored-products pest mainly controlled by insecticides. Spinosad, an environmentally friendly biological insecticide with low mammalian toxicity, is considered a suitable candidate for R. dominica management.
View Article and Find Full Text PDFProximity-Ligation Metagenomic Sequence Analysis Reveals That the Antibiotic Resistome Makes Significant Transitions During Municipal Wastewater Treatment.
Environ Microbiol
January 2025
Department of Civil, Environmental, and Geo-Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Shotgun and proximity-ligation metagenomic sequencing were used to generate thousands of metagenome assembled genomes (MAGs) from the untreated wastewater, activated sludge bioreactors, and anaerobic digesters from two full-scale municipal wastewater treatment facilities. Analysis of the antibiotic resistance genes (ARGs) in the pool of contigs from the shotgun metagenomic sequences revealed significantly different relative abundances and types of ARGs in the untreated wastewaster compared to the activated sludge bioreactors or the anaerobic digesters (p < 0.05).
View Article and Find Full Text PDFInvestigating non-target site resistance to pyroxsulam in a glyphosate-resistant Lolium rigidum population.
Pest Manag Sci
January 2025
College of Plant Protection, Hunan Agricultural University, Changsha, China.
Background: Resistance to multiple herbicides is common in Lolium rigidum. Here, resistance to acetolactate synthase (ALS)- and susceptibility to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides was confirmed in a glyphosate-resistant L. rigidum population (NLR70) from Australia and the mechanisms of pyroxsulam resistance were examined.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!